Metastasis-suppressing NID2, an epigenetically-silenced gene, in the pathogenesis of nasopharyngeal carcinoma and esophageal squamous cell carcinoma.
Oncotarget
; 7(48): 78859-78871, 2016 Nov 29.
Article
em En
| MEDLINE
| ID: mdl-27793011
ABSTRACT
Nidogen-2 (NID2) is a key component of the basement membrane that stabilizes the extracellular matrix (ECM) network. The aim of the study is to analyze the functional roles of NID2 in the pathogenesis of nasopharyngeal carcinoma (NPC) and esophageal squamous cell carcinoma (ESCC). We performed genome-wide methylation profiling of NPC and ESCC and validated our findings using the methylation-sensitive high-resolution melting (MS-HRM) assay. Results showed that promoter methylation of NID2 was significantly higher in NPC and ESCC samples than in their adjacent non-cancer counterparts. Consistently, down-regulation of NID2 was observed in the clinical samples and cell lines of both NPC and ESCC. Re-expression of NID2 suppresses clonogenic survival and migration abilities of transduced NPC and ESCC cells. We showed that NID2 significantly inhibits liver metastasis. Mechanistic studies of signaling pathways also confirm that NID2 suppresses the EGFR/Akt and integrin/FAK/PLCγ metastasis-related pathways. This study provides novel insights into the crucial tumor metastasis suppression roles of NID2 in cancers.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
/
Tipos_de_cancer
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Esofago
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Esofágicas
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Carcinoma
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Carcinoma de Células Escamosas
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Moléculas de Adesão Celular
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Neoplasias Nasofaríngeas
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Metilação de DNA
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Inativação Gênica
Tipo de estudo:
Etiology_studies
Idioma:
En
Revista:
Oncotarget
Ano de publicação:
2016
Tipo de documento:
Article