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Microencapsulation of Hepatocytes and Mesenchymal Stem Cells for Therapeutic Applications.
Meier, Raphael P H; Montanari, Elisa; Morel, Philippe; Pimenta, Joël; Schuurman, Henk-Jan; Wandrey, Christine; Gerber-Lemaire, Sandrine; Mahou, Redouan; Bühler, Leo H.
Afiliação
  • Meier RP; Department of Surgery, Visceral and Transplant Surgery, Geneva University Hospital and Medical School, Rue Gabrielle-Perret-Gentil 4, 1211, Geneva, Switzerland. Raphael.Meier@hcuge.ch.
  • Montanari E; Department of Surgery, Visceral and Transplant Surgery, Geneva University Hospital and Medical School, Rue Gabrielle-Perret-Gentil 4, 1211, Geneva, Switzerland.
  • Morel P; Department of Surgery, Visceral and Transplant Surgery, Geneva University Hospital and Medical School, Rue Gabrielle-Perret-Gentil 4, 1211, Geneva, Switzerland.
  • Pimenta J; Department of Surgery, Visceral and Transplant Surgery, Geneva University Hospital and Medical School, Rue Gabrielle-Perret-Gentil 4, 1211, Geneva, Switzerland.
  • Schuurman HJ; Department of Surgery, Visceral and Transplant Surgery, Geneva University Hospital and Medical School, Rue Gabrielle-Perret-Gentil 4, 1211, Geneva, Switzerland.
  • Wandrey C; Interfaculty Institute of Bioengineering and Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.
  • Gerber-Lemaire S; Interfaculty Institute of Bioengineering and Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.
  • Mahou R; Interfaculty Institute of Bioengineering and Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.
  • Bühler LH; Department of Surgery, Visceral and Transplant Surgery, Geneva University Hospital and Medical School, Rue Gabrielle-Perret-Gentil 4, 1211, Geneva, Switzerland.
Methods Mol Biol ; 1506: 259-271, 2017.
Article em En | MEDLINE | ID: mdl-27830559
Encapsulated hepatocyte transplantation and encapsulated mesenchymal stem cell transplantation are newly developed potential treatments for acute and chronic liver diseases, respectively. Cells are microencapsulated in biocompatible semipermeable alginate-based hydrogels. Microspheres protect cells against antibodies and immune cells, while allowing nutrients, small/medium size proteins and drugs to diffuse inside and outside the polymer matrix. Microencapsulated cells are assessed in vitro and designed for experimental transplantation and for future clinical applications.Here, we describe the protocol for microencapsulation of hepatocytes and mesenchymal stem cells within hybrid poly(ethylene glycol)-alginate hydrogels.
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Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Cápsulas / Hepatócitos / Transplante de Células-Tronco Mesenquimais / Composição de Medicamentos / Doença Hepática Terminal Tipo de estudo: Guideline Limite: Animals / Humans Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Suíça
Buscar no Google
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PubMed Links

Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Cápsulas / Hepatócitos / Transplante de Células-Tronco Mesenquimais / Composição de Medicamentos / Doença Hepática Terminal Tipo de estudo: Guideline Limite: Animals / Humans Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Suíça