Development and validation of a near infrared spectrophotometric method to determine total antioxidant activity of milk.
Food Chem
; 220: 371-376, 2017 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-27855913
ABSTRACT
In the present study a spectrophotometric method for the determination of total antioxidant activity (TAA) based on ABTS assay was developed and validated on raw milk (RM), whole UHT milk (WUM), partially skimmed UHT milk (SUM), whole pasteurised milk (WM) and partially skimmed pasteurised milk (SM). The most suitable solvent for antioxidant extraction was 80% acetone. Regardless of the type of milk, the coefficient of determination from the linearity test was greater than 0.95. The limit of detection ranged from 0.74 to 6.07µmoll-1 Trolox equivalents. Repeatability, calculated as relative standard deviation of twenty measurements within a day, and reproducibility, calculated as relative standard deviation of sixty measurements across three days, ranged from 1.24 to 4.04% and from 2.18 to 3.52%, respectively. Preservative added to RM had negligible effects on the TAA of milk. The greatest TAA was measured for SM followed by SUM, RM, WM and WUM.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Alimentacao
Base de dados:
MEDLINE
Assunto principal:
Espectrofotometria
/
Leite
/
Antioxidantes
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
Food Chem
Ano de publicação:
2017
Tipo de documento:
Article