Optimization of SNAP-25-derived peptide substrate for improved detection of botulinum A in the Endopep-MS assay.
Anal Biochem
; 528: 34-37, 2017 07 01.
Article
em En
| MEDLINE
| ID: mdl-28450105
ABSTRACT
Botulinum neurotoxins (BoNTs) are the most toxic proteins in nature. Endopeptidase-mass-spectrometry (Endopep-MS) is used as a specific and rapid in-vitro assay to detect BoNTs. In this assay, immunocaptured toxin cleaves a serotype-specific-peptide-substrate, and the cleavage products are then detected by MS. Here we describe the design of a new peptide substrate for improved detection of BoNT type A (BoNT/A). Our strategy was based on reported BoNT/A-SNAP-25 interactions integrated with analysis method efficiency considerations. Integration of the newly designed substrate led to a 10-fold increase in the assay sensitivity both in buffer and in clinically relevant samples.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Espectrometria de Massas
/
Toxinas Botulínicas Tipo A
/
Proteína 25 Associada a Sinaptossoma
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Israel