Antimicrobial, anticancer, and antioxidant compounds from Premna resinosa growing in Saudi Arabia.
Pharm Biol
; 55(1): 1759-1766, 2017 Dec.
Article
em En
| MEDLINE
| ID: mdl-28508699
ABSTRACT
CONTEXT Premna resinosa (Hochst.) Schauer (Lamiaceae) is used in many places to treat bronchitis, respiratory illness and convulsions of the rib cage. OBJECTIVE:
This study evaluates the anticancer, antimicrobial and antioxidant activities of P. resinosa, and isolates some responsible constituents. MATERIALS ANDMETHODS:
The methanol extract of P. resinosa aerial parts and its fractions (n-hexane, dichloromethane, ethyl acetate and n-butanol) were tested. Antimicrobial activity was tested using microdilution method against three Gram-positive and four Gram-negative bacteria. The tested concentrations ranged from 4000 to 7.8 µg/mL and MIC values were determined after 24 h incubation. Anticancer activity was evaluated against three human cancer cell lines (Daoy, HepG2 and SK-MEL28) using MTT assay. Antioxidant activity was investigated by DPPH scavenging method and ß-carotene-linoleic acid assay.RESULTS:
The greatest antimicrobial activity was exhibited by n-hexane fraction (MIC 10 µg/mL) against Staphylococcus aureus, Enterococcus faecalis, and Shigella flexneri. The n-hexane fraction induced the greatest cytotoxic activity against Daoy, HepG2, and SK-MEL28 cell lines with IC50 values of 9.0, 8.5 and 13.2, respectively. Moreover, the dichloromethane and ethyl acetate fractions showed the highest antioxidant potential. A bioassay-guided fractionation led to the isolation and characterization of seven compounds for the first time, namely, quercetin (1), 3-methoxy quercetin (2), kaempferol (3), 3-methoxy kaempferol (4), myricetin 3,7,3'-trimethyl ether (5), lupeol (6), and stigmasterol (7).CONCLUSION:
Our results indicate that P. resinosa is a source for antimicrobial and cytotoxic compounds. However, further work is required to isolate other active principles and to determine the mechanism of action.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Extratos Vegetais
/
Lamiaceae
/
Antibacterianos
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Antineoplásicos Fitogênicos
/
Antioxidantes
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
En
Revista:
Pharm Biol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Arábia Saudita