MEIS homeodomain proteins facilitate PARP1/ARTD1-mediated eviction of histone H1.
J Cell Biol
; 216(9): 2715-2729, 2017 09 04.
Article
em En
| MEDLINE
| ID: mdl-28739678
ABSTRACT
Pre-B-cell leukemia homeobox (PBX) and myeloid ecotropic viral integration site (MEIS) proteins control cell fate decisions in many physiological and pathophysiological contexts, but how these proteins function mechanistically remains poorly defined. Focusing on the first hours of neuronal differentiation of adult subventricular zone-derived stem/progenitor cells, we describe a sequence of events by which PBX-MEIS facilitates chromatin accessibility of transcriptionally inactive genes In undifferentiated cells, PBX1 is bound to the H1-compacted promoter/proximal enhancer of the neuron-specific gene doublecortin (Dcx) Once differentiation is induced, MEIS associates with chromatin-bound PBX1, recruits PARP1/ARTD1, and initiates PARP1-mediated eviction of H1 from the chromatin fiber. These results for the first time link MEIS proteins to PARP-regulated chromatin dynamics and provide a mechanistic basis to explain the profound cellular changes elicited by these proteins.
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Neuropeptídeos
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Cromatina
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Histonas
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Proteínas de Homeodomínio
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Linhagem da Célula
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Células-Tronco Adultas
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Neurogênese
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Células-Tronco Neurais
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Poli(ADP-Ribose) Polimerase-1
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
J Cell Biol
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Alemanha