Transplantation of Zebrafish Cells by Conventional Pneumatic Microinjector.
Zebrafish
; 15(1): 73-76, 2018 02.
Article
em En
| MEDLINE
| ID: mdl-29023202
ABSTRACT
Generating chimeric zebrafish by transplantation is extremely useful for live imaging in developmental, stem cell, and cancer biology, and to answer the questions of how cells acquire, keep, and/or change their fate. However, as it is technically challenging, the use of transplantation approach remains very limited by the zebrafish community. In this study, we show that this cell grafting operation can be easily achieved by using a conventional pneumatic microinjector normally used for microinjections. Compared with previously published protocols, which need additional transplantation apparatus, this alternative transplantation method works well, but needs a simpler experimental setup, and is more accessible to all investigators.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Peixe-Zebra
/
Genes Reporter
/
Transplante de Células-Tronco
/
Microinjeções
Limite:
Animals
Idioma:
En
Revista:
Zebrafish
Assunto da revista:
BIOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China