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Quantitative assessment of neuropilin-2 as a simple and sensitive diagnostic assay for spitzoid melanocytic lesions.
Eisenstein, Anna; Panova, Izabela P; Chung, Hye J; Goldberg, Lynne J; Zhang, Qing; Lazova, Rossitza; Bhawan, Jag; Busam, Klaus J; Symanowski, James T; Alani, Rhoda M; Ryu, Byungwoo.
Afiliação
  • Eisenstein A; Department of Dermatology, Boston University School of Medicine, Boston, Massachusetts.
  • Panova IP; Department of Dermatology, Boston University School of Medicine, Boston, Massachusetts.
  • Chung HJ; Department of Dermatology, Boston University School of Medicine, Boston, Massachusetts.
  • Goldberg LJ; Department of Dermatology, Boston University School of Medicine, Boston, Massachusetts.
  • Zhang Q; Department of Cancer Biostatistics, Levine Cancer Institute, Carolinas Health Care System, Charlotte, North Carolina.
  • Lazova R; Department of Dermatology, Yale University School of Medicine, New Heaven, Connecticut.
  • Bhawan J; California Skin Institute, San Jose, California, USA.
  • Busam KJ; Department of Dermatology, Boston University School of Medicine, Boston, Massachusetts.
  • Symanowski JT; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York City, New York.
  • Alani RM; Department of Cancer Biostatistics, Levine Cancer Institute, Carolinas Health Care System, Charlotte, North Carolina.
  • Ryu B; Department of Dermatology, Boston University School of Medicine, Boston, Massachusetts.
Melanoma Res ; 28(1): 71-75, 2018 02.
Article em En | MEDLINE | ID: mdl-29227334
There is a significant need for the development of diagnostic tools that can precisely distinguish Spitz nevi and spitzoid melanomas. Here, we report the development of a PCR-based quantitative diagnostic assay for spitzoid melanocytic lesions utilizing the expression ratio of neuropilin-2 and melan-A genes in primary tumor specimens. We find that the expression ratio of neuropilin-2/melan-A is significantly increased in spitzoid melanomas compared with Spitz nevi. The diagnostic potential of this quantitative assay was validated in two independent sets of patient samples as demonstrated in a receiver operating characteristic curve analysis showing an area under the curve value of 91.8%. Furthermore, the assay was found to quantitatively distinguish the clinical nature of atypical spitzoid melanocytic lesions that were diagnostically undetermined using histopathologic criteria alone. Our data indicate that this quantitative assay may be used as a tool in determining the diagnostic classification of histologically challenging spitzoid tumors.
Assuntos

Texto completo: 1 Coleções: 01-internacional Temas: Geral / Tipos_de_cancer / Pele Base de dados: MEDLINE Assunto principal: Biomarcadores Tumorais / Nevo de Células Epitelioides e Fusiformes / Neuropilina-2 / Testes Diagnósticos de Rotina / Melanócitos / Melanoma Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Melanoma Res Assunto da revista: NEOPLASIAS Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Temas: Geral / Tipos_de_cancer / Pele Base de dados: MEDLINE Assunto principal: Biomarcadores Tumorais / Nevo de Células Epitelioides e Fusiformes / Neuropilina-2 / Testes Diagnósticos de Rotina / Melanócitos / Melanoma Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Melanoma Res Assunto da revista: NEOPLASIAS Ano de publicação: 2018 Tipo de documento: Article