Paper-based immunosensor with signal amplification by enzyme-labeled anti-p16INK4a multifunctionalized gold nanoparticles for cervical cancer screening.
Nanomedicine
; 14(3): 1051-1058, 2018 04.
Article
em En
| MEDLINE
| ID: mdl-29407199
ABSTRACT
The aim of this study was to develop a paper-based immunosensor for cervical cancer screening, with signal amplification by multifunctionalized gold nanoparticles (AuNPs). The AuNPs were functionalized with a highly specific antibody to the p16INK4a cancer biomarker. The signal was amplified using a combination of the peroxidase activity of horseradish peroxidase (HRP) enzyme-antibody conjugate and the peroxidase-like activity of the AuNPs. The immune complex of p16INK4a protein and multifunctionalized AuNPs was deposited on the nitrocellulose membrane, and a positive result was generated by catalytic oxidation of peroxidase enzyme substrate 3,3',5,5'-Tetramethylbenzidine (TMB). The entire reaction occurred on the membrane within 30 min. Evaluation in clinical samples revealed 85.2% accuracy with a kappa coefficient of 0.69. This proof of concept study demonstrates the successful development of a highly accurate, paper-based immunosensor that is easy to interpret using the naked eye and that is suitable for cervical cancer screening in low-resource settings.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
/
Saude_da_mulher
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Colo_do_utero
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Tipos_de_cancer
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Colo_do_utero
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Outros_tipos
Base de dados:
MEDLINE
Assunto principal:
Papel
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Técnicas Biossensoriais
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Neoplasias do Colo do Útero
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Inibidor p16 de Quinase Dependente de Ciclina
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Nanopartículas Metálicas
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Detecção Precoce de Câncer
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Ouro
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Peroxidase do Rábano Silvestre
Tipo de estudo:
Diagnostic_studies
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Observational_studies
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Risk_factors_studies
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Screening_studies
Limite:
Female
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Humans
Idioma:
En
Revista:
Nanomedicine
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Tailândia