Neuronalinjury and roles of apoptosis and autophagy in a neonatal rat model of hypoxia-ischemia-induced periventricular leukomalacia.
Mol Med Rep
; 17(4): 5940-5949, 2018 04.
Article
em En
| MEDLINE
| ID: mdl-29436652
ABSTRACT
As research into periventricular leukomalacia (PVL) gradually increases, concerns are emerging about longterm neuron injury. The present study aimed to investigate neuronal injury and the relevant alterations in apoptosis and autophagy in a PVL model established previously. A rat model of hypoxiaischemiainduced PVL was established. In the model group, SpragueDawley (SD) rats [postnatal day 3 (P3)] were subjected to right common carotid artery ligation followed by suturing and exposed to 68% oxygen for 2 h; in the control group, SD rats (P3) were subjected to right common carotid artery dissection followed by suturing, without ligation and hypoxic exposure. At 1, 3, 7 and 14 days following modeling, brain tissue samples were collected and stained with hematoxylin and eosin. Cellular apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and the protein and mRNA expression alterations of neuronal nuclei (NeuN), caspase3 and Beclin 1 in the model group were detected by western blot analysis and reverse transcriptionquantitative polymerase chain reaction (RTqPCR) analyses. Compared with the control group, the protein and mRNA expression levels of NeuN (a marker of mature neurons) were markedly reduced, the number of positive cells was increased as detected by TUNEL, and the protein and mRNA expression levels of caspase3 and Beclin 1 were elevated in the model group. In the rat model of hypoxiaischemiainduced PVL, oligodendrocyte injury and myelinization disorders were observed, in addition to neuron injury, a decrease in mature neurons and the copresence of apoptosis and autophagy. However, apoptosis and autophagy exist in different phases Apoptosis is involved in neuron injury, while autophagy is likely to have a protective role.
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Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Autofagia
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Leucomalácia Periventricular
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Apoptose
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Hipóxia-Isquemia Encefálica
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Neurônios
Limite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2018
Tipo de documento:
Article