Investigating miRNA-661 and ATG4-B mRNA expression as potential biomarkers for hepatocellular carcinoma.

Ali, Mahmoud A; Matboli, Marwa; El-Khazragy, Nashwa; Saber, Osama; El-Nakeep, Sarah; Abdelzaher, Hana M; Shafei, Ayman El-Sayed; Mostafa, Randa

Ali, Mahmoud A; Matboli, Marwa; El-Khazragy, Nashwa; Saber, Osama; El-Nakeep, Sarah; Abdelzaher, Hana M; Shafei, Ayman El-Sayed; Mostafa, Randa.

Afiliação

Ali MA; Department of Biomedical Research, Armed Forces College of Medicine (AFCM), Cairo, 11774, Egypt.
Matboli M; Department of Medical Biochemistry & Molecular Biology, Ain Shams Faculty of Medicine Research Center (Masri), 11778, Eygpt.
El-Khazragy N; Department of Clinical Pathology, Oncology Diagnostic Unit, Faculty of Medicine, Ain Shams University, Cairo, 11778, Egypt.
Saber O; Armed Forces College of Medicine (AFCM), Cairo, 11774, Egypt.
El-Nakeep S; Hepatology & Gastroenterology Unit, Internal Medicine Department, Faculty of Medicine, Ain Shams University, Cairo, 11778, Egypt.
Abdelzaher HM; Faculty of Biotechnology, October University for Modern Sciences & Arts, Cairo, 12585, Egypt.
Shafei AE; Department of Biomedical Research, Armed Forces College of Medicine (AFCM), Cairo, 11774, Egypt.
Mostafa R; Department of Biomedical Research, Armed Forces College of Medicine (AFCM), Cairo, 11774, Egypt.

Biomark Med ; 12(3): 245-256, 2018 03.

Article em En | MEDLINE | ID: mdl-29441798

RESUMO

AIM: We aimed to examine the statistical association between serum expression of miRNA 661 (miR-661) and ATG-4B mRNA and hepatocellular carcinoma (HCC) based on in silico data analysis followed by clinical validation. PATIENTS & METHODS: Quantitative reverse-transcriptase real-time PCR was used to examine the expression of miR-661 and ATG-4B mRNA in the sera of HCC patients versus control. RESULTS: The expression of miR-661 and ATG-4B mRNA was positive in 97.14 and 77.14%, respectively, in HCC patients. The survival analysis showed that ATG-4B mRNA was an independent prognostic factor. CONCLUSION: Our data are the first report of its kind regarding the considerable clinical significance of miR-661 and ATG-4B mRNA in HCC patients.

Assuntos

Proteínas Relacionadas à Autofagia/genética; Biomarcadores Tumorais/genética; Carcinoma Hepatocelular/diagnóstico; Cisteína Endopeptidases/genética; Neoplasias Hepáticas/diagnóstico; MicroRNAs/sangue; RNA Mensageiro/sangue; Área Sob a Curva; Biomarcadores Tumorais/metabolismo; Carcinoma Hepatocelular/genética; Carcinoma Hepatocelular/patologia; Estudos de Casos e Controles; Feminino; Humanos; Neoplasias Hepáticas/genética; Neoplasias Hepáticas/patologia; Masculino; Pessoa de Meia-Idade; Estadiamento de Neoplasias; Prognóstico; Curva ROC; Regulação para Cima

Palavras-chave

ATG-4B; bioinformatics; biomarkers; hepatocellular carcinoma; miR-661; prognosis

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Texto completo: 1 Coleções: 01-internacional Temas: Geral / Tipos_de_cancer / Outros_tipos Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Cisteína Endopeptidases / Biomarcadores Tumorais / Carcinoma Hepatocelular / MicroRNAs / Proteínas Relacionadas à Autofagia / Neoplasias Hepáticas Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Female / Humans / Male / Middle aged Idioma: En Revista: Biomark Med Assunto da revista: BIOQUIMICA / MEDICINA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Egito

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