Infrared Laser Improves Collagen Organization in Muscle and Tendon Tissue During the Process of Compensatory Overload.
Photomed Laser Surg
; 36(3): 130-136, 2018 Mar.
Article
em En
| MEDLINE
| ID: mdl-29466099
ABSTRACT
BACKGROUND:
The photobiomodulation using the low-level laser therapy (LLLT) exerts a positive modulating effect on the synthesis of collagen in skeletal muscles and tendons. However, few studies have addressed this effect during the compensatory overload.OBJECTIVE:
Evaluate the effect of infrared laser on the deposition and organization of collagen fibers in muscle and tendon tissue during compensatory overload of the plantar muscle in rats. MATERIALS ANDMETHODS:
Wistar rats were submitted to bilateral ablation of the synergist muscles of the hind paws and divided in groups Control, Hypertrophy, and Hypertrophy (H)+LLLT (780 nm, 40 mW, 9.6 J/cm2 and 10 s/point, 8 points, total energy 3.2 J, daily), evaluated at 7 and 14 days. Muscle cuts were stained with Picrosirius-Red and hematoxylin-eosin and tendon cuts were submitted to birefringence for determination of collagen distribution and organization.RESULTS:
After 7 days an increase was observed in the area between beam muscles in H+LLLT (25.45% ± 2.56) in comparison to H (20.3% ± 3.31), in mature fibers and fibrilis in H+LLLT (29346.88 µm2 ± 2182.56; 47602.8 µm2 ± 2201.86 respectively) in comparison to H (26656.5 µm2 ± 1880.46; 45630.34 µm2 ± 2805.82 respectively) and in the collagen area in H+LLLT (2.25% ± 0.19) in comparison to H (2.0% ± 0.15). However, after 14 days a reduction was observed in the area between beam muscles in H+LLLT (13.88% ± 2.54) in comparison to H (19.1% ± 2.61), in fibrils and mature fibers in H+LLLT (17174.1 µm2 ± 2563.82; 32634.04 µm2 ± 1689.38 respectively) in comparison to H (55249.86 µm2 ± 1992.65; 44318.36 µm2 ± 1759.57) and in the collagen area in H+LLLT (1.76% ± 0.16) in comparison to H (2.09 ± 0.27). A greater organization of collagen fibers in the tendon was observed after 7 and 14 days in H+LLLT groups.CONCLUSIONS:
Infrared laser irradiation induces an improvement in collagen organization in tendons and a reduction in the total area of collagen in muscles during compensatory atrophy following the ablation of synergist muscles.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Tendões
/
Cicatrização
/
Colágeno
/
Músculo Esquelético
/
Terapia com Luz de Baixa Intensidade
Limite:
Animals
Idioma:
En
Revista:
Photomed Laser Surg
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Brasil