BuShenNingXin decoction suppresses osteoclastogenesis by modulating RANKL/OPG imbalance in the CD4+ T lymphocytes of ovariectomized mice.
Int J Mol Med
; 42(1): 299-308, 2018 Jul.
Article
em En
| MEDLINE
| ID: mdl-29717766
ABSTRACT
Postmenopausal osteoporosis (PMO) has been recognized as an inflammatory condition. CD4+ T cells serve a key role in the interaction between bone metabolism and the immune system. BuShenNingXin decoction (BSNXD), a traditional Chinese medicine, has been ultilized as a remedy for PMO. In the present study, the aim was to investigate the immune modulatory effects of BSNXD on CD4+ T cells, receptor activation of nuclear factor κB ligand (RANKL)/osteoprotegerin (OPG) imbalance, skeletal parameters and osteoclastogenesis. Ovariectomized (OVX) mice were treated with a series of concentrations of BSNXD and then autopsied. The bone phenotype was analyzed by micro computed tomography. CD4+ T cells were isolated and their percentage was measured using flow cytometry (FCM). RANKL and OPG expression by the CD4+ T cells at the transcriptional and translational levels were quantified by reverse transcription-quantitative polymerase chain reaction, ELISA and FCM. CD4+ T cells were cultured with blood serum derived from BSNXDtreated OVX mice (BSNXDderived serum) and the apoptosis rate was quantified by FCM. CD4+ T cells were co-cultured with bone marrowderived macrophages and exposed to BSNXDderived serum to whether CD4+ T cells are involved in BSNXDmodulated osteoclastogenesis and the results were quantified via tartrateresistant acid phosphatase staining. The results revealed that BSNXD ameliorated OVXinduced bone loss, prevented the expansion of CD4+ T cells and restored the RANKL/OPG imbalance in the CD4+ T cells of OVX mice. In vitro, BSNXDderived serum promoted the apoptosis of CD4+ T cells. The coculture system demonstrated that CD4+ T cells from OVX mice increase osteoclastogenesis, while this effect was suppressed by BSNXD administration. The findings of the study collectively suggest that BSNXD exerts an immunoprotective effect on the bone phenotype of OVX mice by ameliorating RANKL/OPG imbalance in CD4+ T cells and attenuating osteoclastogenesis.
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Osteoclastos
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Osteogênese
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Medicamentos de Ervas Chinesas
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Ovariectomia
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Linfócitos T CD4-Positivos
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Ligante RANK
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Osteoprotegerina
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Int J Mol Med
Assunto da revista:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Ano de publicação:
2018
Tipo de documento:
Article