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[Effects of Xialiqi Capsules on the expressions of PCNA and caspase-3 in rats withbenign prostatic hyperplasia].
Cai, Hong-Cai; Song, Le-Bin; Zhang, Guo-Wei; Qing, Xing-Rong; Mo, Dun-Sheng; Liu, Wei; Zhan, Xu-Xin; Huang, Yu-Feng; Shang, Xue-Jun.
Afiliação
  • Cai HC; Department of Andrology, Jinling Hospital Affiliated to Southern Medical University / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China.
  • Song LB; First Clinical Medical College of Nanjing Medical University, Nanjing, Jiangsu 210009, China.
  • Zhang GW; Department of Andrology, Jinling Hospital Affiliated to Southern Medical University / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China.
  • Qing XR; Family Planning Research Institute/Center of Reproductive Medicine, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
  • Mo DS; Department of Urology, Liuzhou Workers' Hospital / The Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi 545005, China.
  • Liu W; Department of Andrology, Jinling Hospital Affiliated to Southern Medical University / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China.
  • Zhan XX; Department of Reproductive Medicine, Xi'an No.4 Hospital, Xi'an, Shaanxi 710004, China.
  • Huang YF; Department of Andrology, Jinling Hospital Affiliated to Nanjing University School of Medicine / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China.
  • Shang XJ; Department of Andrology, Jinling Hospital Affiliated to Southern Medical University / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China.
Zhonghua Nan Ke Xue ; 23(8): 728-733, 2017 Aug.
Article em Zh | MEDLINE | ID: mdl-29726649
OBJECTIVE: To explore the effects of Xialiqi Capsules(XLQ) on the expressions of the proliferating cell nuclear antigen (PCNA) and caspase-3 in the prostate tissue of the BPH rat model. METHODS: Fifty male SD ratswereequally randomized into groups A (sham operation control), B (BPH model control), C (high-dose XLQ), D (low-dose XLQ), and E (finasteridecontrol) andthe BPH modelswere established by subcutaneous injection of testosterone propionate at 0.5 mg per kilogram of the body weight per day for 30 days after castration. After modeling, the animals in groups A and B were treated intragastricallywith normal saline, while those in C, D, and E with XLQ at 1.20 and 0.61 g per kilogram of the body weight per day or finasterideat 0.8 mg per kilogram of the body weight per day, respectively, all for 30 days. Then,the bilateral prostates were harvestedfrom the rats for calculation of the prostatic index (prostate wet weight/ body weight) and determination of the expressions of PCNA and caspase-3 in the prostate tissue by immunohistochemistry and immunofluorescence staining, respectively. RESULTS: The prostate wet weight and prostate index were significantly increased in group B as compared with group A, (ï¼»1326±60ï¼½ vsï¼»471±17ï¼½ g, P<0.01; ï¼»2.89±0.18ï¼½ vs ï¼»1.06±0.06ï¼½ mg/g, P<0.01), but decreased in groups C (ï¼»914±36ï¼½ g;ï¼»2.02±0.08ï¼½ mg/g), D (ï¼»1 099±46ï¼½g;ï¼»2.39±0.11ï¼½ mg/g), and E (ï¼»817±53ï¼½ g;ï¼»1.83±0.10ï¼½ mg/g)in comparison with B (P<0.01), with statistically significant differences among groups C, D, and E(P<0.01) and most significantly in E.The PCNA level in the prostate tissue wasremarkably higher in group B than in A, but lower in groups C, D and E than in B. The expression of caspase-3 was down-regulatedin group B as compared with A, but up-regulated in groups C, D and E in comparison with B, most significantly in E. CONCLUSIONS: Xialiqi Capsules can effectively reduce the prostate wet weight and prostatic index of in rats with BPH by inhibiting the level of PCNA and promoting the expression of caspase-3.
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Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Próstata / Hiperplasia Prostática / Medicamentos de Ervas Chinesas / Antígeno Nuclear de Célula em Proliferação / Caspase 3 Limite: Animals Idioma: Zh Revista: Zhonghua Nan Ke Xue Assunto da revista: MEDICINA REPRODUTIVA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China
Buscar no Google
Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Próstata / Hiperplasia Prostática / Medicamentos de Ervas Chinesas / Antígeno Nuclear de Célula em Proliferação / Caspase 3 Limite: Animals Idioma: Zh Revista: Zhonghua Nan Ke Xue Assunto da revista: MEDICINA REPRODUTIVA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China