Uncovering Bistability in the Rac1/RhoA Signaling Network Through Integrating Computational Modeling and Experimentation.
Methods Mol Biol
; 1821: 21-36, 2018.
Article
em En
| MEDLINE
| ID: mdl-30062402
ABSTRACT
The members of the Rho family of small guanosine triphosphatases (GTPases), Rac1 and RhoA, play critical roles in the regulation of cell migration, actin dynamics, and cytoskeletal system. It has been long known that a mutual inhibition relationship exists between Rac1 and RhoA, and the Rac1/RhoA circuitry has been theoretically predicted to be capable of displaying bistability, a phenomenon whereby a system could settle in either one of the two stable steady states. However, it was only until recently that bistable behavior was demonstrated experimentally both at the biochemical and cellular phenotypic levels, through an integrative approach combining computational modeling and wet-lab experimentation. Here, we describe how such systems biology approaches could be employed to uncover bistability and its hallmark features, using the Rac1/RhoA network as an illustrative example. This may provide guidance for future work aimed at identifying bistable behaviors in other cellular processes.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Simulação por Computador
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Transdução de Sinais
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Proteínas rac1 de Ligação ao GTP
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Proteína rhoA de Ligação ao GTP
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Modelos Biológicos
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Reino Unido