Intracellular RNA-tracking methods.
Open Biol
; 8(10)2018 10 03.
Article
em En
| MEDLINE
| ID: mdl-30282659
ABSTRACT
RNA tracking allows researchers to visualize RNA molecules in cells and tissues, providing important spatio-temporal information regarding RNA dynamics and function. Methods such as fluorescent in situ hybridization (FISH) and molecular beacons rely on complementary oligonucleotides to label and view endogenous transcripts. Other methods create artificial chimeric transcripts coupled with bacteriophage-derived coat proteins (e.g. MS2, λN) to tag molecules in live cells. In other approaches, endogenous RNAs are recognized by complementary RNAs complexed with noncatalytic Cas proteins. Each technique has its own set of strengths and limitations that must be considered when planning an experiment. Here, we discuss the mechanisms, advantages, and weaknesses of in situ hybridization, molecular beacons, MS2 tagging and Cas-derived systems, as well as how RNA tracking can be employed to study various aspects of molecular biology.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Hibridização in Situ Fluorescente
/
RNA não Traduzido
/
Análise de Célula Única
/
Sistemas CRISPR-Cas
Limite:
Humans
Idioma:
En
Revista:
Open Biol
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Estados Unidos