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Anti-silencing factor Epe1 associates with SAGA to regulate transcription within heterochromatin.
Bao, Kehan; Shan, Chun-Min; Moresco, James; Yates, John; Jia, Songtao.
Afiliação
  • Bao K; Department of Biological Sciences, Columbia University, New York, New York 10027, USA.
  • Shan CM; Department of Biological Sciences, Columbia University, New York, New York 10027, USA.
  • Moresco J; Department of Molecular Medicine and Neurobiology, The Scripps Research Institute, La Jolla, California 92037, USA.
  • Yates J; Department of Molecular Medicine and Neurobiology, The Scripps Research Institute, La Jolla, California 92037, USA.
  • Jia S; Department of Biological Sciences, Columbia University, New York, New York 10027, USA.
Genes Dev ; 33(1-2): 116-126, 2019 01 01.
Article em En | MEDLINE | ID: mdl-30573453
ABSTRACT
Heterochromatin is a highly condensed form of chromatin that silences gene transcription. Although high levels of transcriptional activities disrupt heterochromatin, transcription of repetitive DNA elements and subsequent processing of the transcripts by the RNAi machinery are required for heterochromatin assembly. In fission yeast, a JmjC domain protein, Epe1, promotes transcription of DNA repeats to facilitate heterochromatin formation, but overexpression of Epe1 leads to heterochromatin defects. However, the molecular function of Epe1 is not well understood. By screening the fission yeast deletion library, we found that heterochromatin defects associated with Epe1 overexpression are alleviated by mutations of the SAGA histone acetyltransferase complex. Overexpressed Epe1 associates with SAGA and recruits SAGA to heterochromatin regions, which leads to increased histone acetylation, transcription of repeats, and the disruption of heterochromatin. At its normal expression levels, Epe1 also associates with SAGA, albeit weakly. Such interaction regulates histone acetylation levels at heterochromatin and promotes transcription of repeats for heterochromatin assembly. Our results also suggest that increases of certain chromatin protein levels, which frequently occur in cancer cells, might strengthen relatively weak interactions to affect the epigenetic landscape.
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Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Schizosaccharomyces / Proteínas Nucleares / Heterocromatina / Regulação Fúngica da Expressão Gênica / Proteínas de Schizosaccharomyces pombe Tipo de estudo: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Genes Dev Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Schizosaccharomyces / Proteínas Nucleares / Heterocromatina / Regulação Fúngica da Expressão Gênica / Proteínas de Schizosaccharomyces pombe Tipo de estudo: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Genes Dev Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Estados Unidos