Evolutionary interplay of single nucleotide polymorphisms at the promoter region of TNF-α gene in different clinical outcomes of malaria in India.
Infect Genet Evol
; 69: 107-116, 2019 04.
Article
em En
| MEDLINE
| ID: mdl-30677532
ABSTRACT
Host genetic factors are frequently ascribed to differential malaria outcomes as a by-product of evolutionary adaptation. To this respect, Tumor Necrosis factor alpha (TNF-α), a human cytokine, is known to be associated with malaria through its differential regulation in diverse malaria manifestations. Since diversity in differential malaria outcome is uncommon in every endemic settings, possible association of TNF-α and malaria is not commonly established. In order to check for association between the occurrence of Single Nucleotide Polymorphisms (SNPs) in the TNF-α gene with different malaria manifestations, we have sequenced a 4011â¯bp region constituting the promoter and the whole gene of human TNF-α in 61 patients [(16 cerebral plus severe (SCM), 21 severe (SM) and 24 uncomplicated (UM)] samples in a highly malaria endemic state (Odisha) of India. Multiple sequence alignment revealed presence of six SNPs (-1031â¯Tâ¯>â¯C, -863Câ¯>â¯A, -857Câ¯>â¯T, -308Gâ¯>â¯A, -806Câ¯>â¯T, +787Câ¯>â¯A), out of which the -806Câ¯>â¯T and +787Câ¯>â¯A are novel in malaria patients in general and the +787Câ¯>â¯A was detected for the first time in humans. Although alleles due to six different SNPs segregate differentially in the three groups of malaria (SCM, SM and UM) in the present study, interestingly, for the -1031â¯Tâ¯>â¯C position, the frequency of individuals possessing the homozygous rare allele was higher in the SCM group with a higher number of heterozygotes in the UM group. The Tajima's D values considering all the SNPs in a defined group were positive and statistically insignificant conforming no evolutionary constraint. However, statistically significant deviation from expectation under Hardy-Weinberg equilibrium for -1031â¯Tâ¯>â¯C SNP in the UM group points towards the probable role of natural selection providing some kind of protection to malaria in Odisha, India.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Regiões Promotoras Genéticas
/
Fator de Necrose Tumoral alfa
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Evolução Molecular
/
Predisposição Genética para Doença
/
Polimorfismo de Nucleotídeo Único
/
Malária
Limite:
Female
/
Humans
/
Male
País/Região como assunto:
Asia
Idioma:
En
Revista:
Infect Genet Evol
Assunto da revista:
BIOLOGIA
/
DOENCAS TRANSMISSIVEIS
/
GENETICA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Índia