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Fast enantioseparation of indole phytoalexins in additive free supercritical fluid chromatography.
Kozlov, Oleksandr; Kalíková, Kveta; Gondová, Tatána; Budovská, Mariana; Salayová, Aneta; Tesarová, Eva.
Afiliação
  • Kozlov O; Department of Analytical Chemistry, Faculty of Science, P. J. Safárik University, Moyzesova 11, 040 01 Kosice, Slovak Republic.
  • Kalíková K; Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Hlavova 8, 12843, Prague, Czech Republic. Electronic address: kalikova@natur.cuni.cz.
  • Gondová T; Department of Analytical Chemistry, Faculty of Science, P. J. Safárik University, Moyzesova 11, 040 01 Kosice, Slovak Republic.
  • Budovská M; Department of Organic Chemistry, Faculty of Science, P. J. Safárik University, Moyzesova 11, 040 01 Kosice, Slovak Republic.
  • Salayová A; Department of Organic Chemistry, Faculty of Science, P. J. Safárik University, Moyzesova 11, 040 01 Kosice, Slovak Republic; Department of Chemistry, Biochemistry and Biophysics, Institute of Pharmaceutical Chemistry, University of Veterinary Medicine and Pharmacy, 041 81 Kosice, Slovak Republic.
  • Tesarová E; Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Hlavova 8, 12843, Prague, Czech Republic.
J Chromatogr A ; 1596: 209-216, 2019 Jul 05.
Article em En | MEDLINE | ID: mdl-30910386
A series of chiral indole phytoalexins with potential anticancer and antimicrobial activity were enantioseparated in supercritical fluid chromatography. Two polysaccharide-based chiral stationary phases composed of tris-(3,5-dimethylphenylcarbamate) derivatives of amylose or cellulose coated on 2.5 µm silica particles were successfully used. The influences of the polysaccharide backbone, co-solvent type and co-solvent content in the mobile phase on retention, enantioselectivity and enantioresolution of indole phytoalexins were investigated. Fast baseline separations were achieved for 26 from 27 tested compounds. Amylose-based chiral stationary phase provided higher number of baseline resolutions of the indole phytoalexins than the cellulose-based one. However, certain complementary enantioresolution results towards the studied compounds were observed between the investigated columns. The relationship between structure of the indole phytoalexins and their chromatographic behavior in supercritical fluid chromatography was discussed.
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Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Sesquiterpenos / Técnicas de Química Analítica / Cromatografia com Fluido Supercrítico / Indóis Idioma: En Revista: J Chromatogr A Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Sesquiterpenos / Técnicas de Química Analítica / Cromatografia com Fluido Supercrítico / Indóis Idioma: En Revista: J Chromatogr A Ano de publicação: 2019 Tipo de documento: Article