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Differential expression profiling of long non-coding RNA GAS5 and miR-126-3p in human cancer cells in response to sorafenib.
Faranda, Teresa; Grossi, Ilaria; Manganelli, Michele; Marchina, Eleonora; Baiocchi, Gianluca; Portolani, Nazario; Crosatti, Marialuisa; De Petro, Giuseppina; Salvi, Alessandro.
Afiliação
  • Faranda T; Department of Molecular and Translational Medicine, Division of Biology and Genetics, University of Brescia, Brescia, Italy.
  • Grossi I; Department of Molecular and Translational Medicine, Division of Biology and Genetics, University of Brescia, Brescia, Italy.
  • Manganelli M; Department of Molecular and Translational Medicine, Division of Biology and Genetics, University of Brescia, Brescia, Italy.
  • Marchina E; Department of Molecular and Translational Medicine, Division of Biology and Genetics, University of Brescia, Brescia, Italy.
  • Baiocchi G; Department of Clinical and Experimental Sciences, Surgical Clinic, University of Brescia, Brescia, Italy.
  • Portolani N; Department of Clinical and Experimental Sciences, Surgical Clinic, University of Brescia, Brescia, Italy.
  • Crosatti M; Department of Respiratory Sciences, University of Leicester, Leicester, UK.
  • De Petro G; Department of Molecular and Translational Medicine, Division of Biology and Genetics, University of Brescia, Brescia, Italy.
  • Salvi A; Department of Molecular and Translational Medicine, Division of Biology and Genetics, University of Brescia, Brescia, Italy. alessandro.salvi@unibs.it.
Sci Rep ; 9(1): 9118, 2019 06 24.
Article em En | MEDLINE | ID: mdl-31235746
Long non-coding RNAs (lncRNAs) and microRNAs are involved in numerous physio-pathological conditions included cancer. To better understand the molecular mechanism of the oral antitumor multikinase inhibitor sorafenib, we profiled the expression of a panel of lncRNAs and miRNAs by qPCR array in a sorafenib-treated hepatocellular carcinoma (HCC) cell line. Among the most affected ncRNAs, we found that sorafenib mediated the dysregulation of the lncRNAs GAS5, HOTTIP and HOXA-AS2 and the miR-126-3p, in a panel of human cancer cell lines (HCC, renal and breast carcinomas). By luciferase gene reporter assay, we discovered that GAS5 may act as a sponge for miR-126-3p in HCC cells. The expression level of GAS5 and miR-126-3p was verified in human liquid and/or solid biopsies from HCC patients. miR-126-3p expression in HCC tissues was decreased respect to their correspondent peritumoral tissues. The levels of plasmatic circulating miR-126-3p and GAS5 were significantly higher and lower in HCC patients compared to healthy subjects, respectively. This study highlighted the capability of sorafenib to modulate the expression of a wide range of ncRNAs and specifically, GAS5 and miR-126-3p were involved in the response to sorafenib of different cancer cell types.
Assuntos

Texto completo: 1 Coleções: 01-internacional Temas: Geral / Prevencao_e_fatores_de_risco / Agentes_cancerigenos / Tipos_de_cancer / Outros_tipos Base de dados: MEDLINE Assunto principal: Carcinoma Hepatocelular / Perfilação da Expressão Gênica / MicroRNAs / RNA Longo não Codificante / Sorafenibe / Neoplasias Hepáticas Limite: Humans Idioma: En Revista: Sci Rep Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Itália

Texto completo: 1 Coleções: 01-internacional Temas: Geral / Prevencao_e_fatores_de_risco / Agentes_cancerigenos / Tipos_de_cancer / Outros_tipos Base de dados: MEDLINE Assunto principal: Carcinoma Hepatocelular / Perfilação da Expressão Gênica / MicroRNAs / RNA Longo não Codificante / Sorafenibe / Neoplasias Hepáticas Limite: Humans Idioma: En Revista: Sci Rep Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Itália