Radioprotective properties of food colorant sodium copper chlorophyllin on human peripheral blood cells in vitro.
Mutat Res Genet Toxicol Environ Mutagen
; 845: 403027, 2019 Sep.
Article
em En
| MEDLINE
| ID: mdl-31561900
ABSTRACT
Sodium copper chlorophyllin (CHL) is a food colorant that exhibits many beneficial properties, including potential for use in radiotherapy. Nevertheless, genotoxicity studies investigating radioprotective properties against γ-radiation on human cells are rather scarce. The aim of this study was to assess the cytotoxicity, genotoxicity and induction of malondialdehyde formation on CHL pre-treated whole blood cells after an absorbed dose of 5 Gy γ-radiation. Irradiated whole blood cells pre-treated with 100, 500, and 1000 µg/mL CHL showed less DNA-strand breaks (10.92 ± 0.74%, 10.69 ± 0.68%, and 8.81 ± 0.69%, respectively) than untreated irradiated cells (12.58 ± 0.88%). At the same time, the level of malondialdehyde was lower in CHL pre-treated samples with 100, 500, and 1000 µg/mL CHL (14.11 ± 0.43, 16.35 ± 2.82, and 13.08 ± 1.03 µmol/L, respectively) compared to untreated irradiated samples (24.11 ± 0.25 µmol/L). Regarding cytotoxicity, no changes were observed in the samples tested. Another important finding is that CHL had no cyto/genotoxic properties toward human blood cells. Taken together, since CHL had no cyto/genotoxic effects and showed good radioprotective properties in human blood cells, further studies should be conducted in order to find its possible application in radiotherapy.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Protetores contra Radiação
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Dano ao DNA
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Linfócitos
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Clorofilídeos
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Corantes de Alimentos
Limite:
Adult
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Humans
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Male
Idioma:
En
Revista:
Mutat Res Genet Toxicol Environ Mutagen
Ano de publicação:
2019
Tipo de documento:
Article