The effect of BACE1-AS on ß-amyloid generation by regulating BACE1 mRNA expression.
BMC Mol Biol
; 20(1): 23, 2019 10 01.
Article
em En
| MEDLINE
| ID: mdl-31570097
ABSTRACT
BACKGROUND:
The BACE1 antisense transcript (BACE1-AS) is a conserved long noncoding RNA (lncRNA). The level of BACE1-AS is significantly increased and the level of the BACE1 mRNA is slightly increased in subjects with AD. BACE1-AS exerts a significant moderating effect on the expression of the BACE1 mRNA and promotes the formation of Aß. After the administration of Aß1-42 to SH-SY5Y cells and C57/BL6J mice, we detected the expression of BACE1-AS, BACE1 mRNA, and BACE1 protein, as well as the concentration of Aß1-40. Then, we silenced the expression of BACE1-AS in SH-SY5Y and 20E2 cells using siRNAs targeting BACE1-AS and detected its effects on the levels of the BACE1 mRNA and BACE1 protein and Aß1-40 generation.RESULTS:
The administration of Aß1-42 increased the expression of BACE1-AS, BACE1 mRNA and protein, as well as the concentration of Aß1-40 in SH-SY5Y cells and the brains of C57BL/6J mice. Pretreatment with the BACE1-AS siRNA inhibited the effect of Aß1-42 on increasing the expression of BACE1-AS and BACE1, as well as the generation of Aß.CONCLUSIONS:
The mechanism by which exogenous Aß1-42 induces BACE1 expression and Aß generation is mediated by BACE1-AS. BACE1-AS is involved in the mechanism regulating BACE1 expression and Aß generation in APPsw transgenic cells.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
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Encéfalo
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Peptídeos beta-Amiloides
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Ácido Aspártico Endopeptidases
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Secretases da Proteína Precursora do Amiloide
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RNA Longo não Codificante
Limite:
Animals
/
Humans
Idioma:
En
Revista:
BMC Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
China