Probing Protein-Protein Interactions with Label-Free Mass Spectrometry Quantification in Combination with Affinity Purification by Spin-Tip Affinity Columns.

Liu, Guizhen; Fu, Tao; Han, Ying; Hu, Shichen; Zhang, Xuepei; Zheng, Mengmeng; Hao, Piliang; Pan, Lifeng; Kang, Jingwu

Liu, Guizhen; Fu, Tao; Han, Ying; Hu, Shichen; Zhang, Xuepei; Zheng, Mengmeng; Hao, Piliang; Pan, Lifeng; Kang, Jingwu.

Afiliação

Liu G; State Key Laboratory of Bioorganic and Natural Products Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, China.
Fu T; School of Physical Science and Technology, ShanghaiTech University, Haike Road 100, Shanghai 200120, China.
Han Y; University of Chinese Academy of Sciences, Beijing, China.
Hu S; State Key Laboratory of Bioorganic and Natural Products Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, China.
Zhang X; School of Life Science and Technology, ShanghaiTech University, Haike Road 100, Shanghai 200120, China.
Zheng M; State Key Laboratory of Bioorganic and Natural Products Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, China.
Hao P; State Key Laboratory of Bioorganic and Natural Products Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, China.
Pan L; State Key Laboratory of Bioorganic and Natural Products Chemistry, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, China.
Kang J; University of Chinese Academy of Sciences, Beijing, China.

Anal Chem ; 92(5): 3913-3922, 2020 03 03.

Article em En | MEDLINE | ID: mdl-31992042

Assuntos

Cromatografia de Afinidade/métodos; Mapas de Interação de Proteínas/fisiologia; Proteínas Recombinantes de Fusão/metabolismo; Cromatografia Líquida de Alta Pressão; Histidina/química; Histidina/genética; Histidina/metabolismo; Humanos; Proteínas Associadas aos Microtúbulos/química; Proteínas Associadas aos Microtúbulos/metabolismo; Ácido Nitrilotriacético/química; Oligopeptídeos/química; Oligopeptídeos/genética; Oligopeptídeos/metabolismo; Peptídeos/análise; Ligação Proteica; Proteômica/métodos; Proteínas Recombinantes de Fusão/biossíntese; Proteínas Recombinantes de Fusão/isolamento & purificação; Espectrometria de Massas em Tandem

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Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Proteínas Recombinantes de Fusão / Cromatografia de Afinidade / Mapas de Interação de Proteínas Limite: Humans Idioma: En Revista: Anal Chem Ano de publicação: 2020 Tipo de documento: Article País de afiliação: China

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