Hypoxia-induced USP22-BMI1 axis promotes the stemness and malignancy of glioma stem cells via regulation of HIF-1α.
Life Sci
; 247: 117438, 2020 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-32070708
ABSTRACT
AIMS:
This study intends to investigate the mechanisms of ubiqutin-specific protease 22 (USP22)/B cell-specific Moloney murine leukemia virus integration site 1 (BMI1) on the biological phenotypes of glioma stem cells (GSCs) under hypoxia. MAINMETHODS:
Western blot, Cell Counting Kit-8, colony formation and flow cytometry assays were preformed to evaluate cells biological behaviors. Luciferase assay was utilized to identify the associations among USP22, HIF-1α and BMI1. KEYFINDINGS:
Silencing USP22 reduced the stemness and proliferation of GSCs, and increased its apoptosis in response to hypoxia. Whilst, overexpression of BMI1 reversed these phenomena. Whilst, a significant decrease in proliferation and stemness of GSCs caused by HIF-1α exhaustion were inversed by overexpression of USP22 or BMI1.SIGNIFICANCE:
Function of USP22-BMI1 on biological behaviors of GSCs was regulated by HIF-1α in response to hypoxia.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
/
Tipos_de_cancer
/
Outros_tipos
Base de dados:
MEDLINE
Assunto principal:
Ubiquitina Tiolesterase
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Subunidade alfa do Fator 1 Induzível por Hipóxia
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Complexo Repressor Polycomb 1
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Glioma
/
Hipóxia
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Life Sci
Ano de publicação:
2020
Tipo de documento:
Article