A chemical screening method for menaquinone-producing strains based on HPLC-UV technology.
J Microbiol Methods
; 172: 105907, 2020 05.
Article
em En
| MEDLINE
| ID: mdl-32240706
ABSTRACT
Despite menaquinones (MKs)-4 and - 7 being known to have extensive biological activities and applications, less attention has been paid to the other MKs. Thus, to obtain a range of MKs to further explore their pharmacological activities, structure-activity relationships, and applications, a chemical screening method for MK-producing strains was established based on high-performance liquid chromatography-ultraviolet (HPLC-UV) technology. Considering that Bacillus strains have proven to be an important MK-producing bioresource, twenty-nine putative Bacillus isolates previously sought from a fermented soybean sample were used for the validation of the chemical screening method, which ultimately led to the discovery of sixteen MK-producing strains. Among them, Bacillus subtilis DC-1 presented excellent ability to produce MKs. Another, a purchased strain of B. amyloliquefaciens was discovered to be an MK-producing strain. These results indicated this screening method was simple and highly efficient for the discovery of MK-producing strains, especially those producing a range of MK structures.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Bacillus
/
Programas de Rastreamento
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Cromatografia Líquida de Alta Pressão
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Vitamina K 2
Tipo de estudo:
Diagnostic_studies
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Screening_studies
Idioma:
En
Revista:
J Microbiol Methods
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
China