Studies of metabolites in diarrheal stool specimens positive for Klebsiella, Serratia, and Proteus spp. by frequency-pulsed electron-capture gas chromatography.

ABSTRACT

Diarrheal stools from infants from which Klebsiella pneumoniae, Serratia liquefaciens, and Proteus mirabilis were isolated as possible causative agents of diarrhea were studied. These stools, along with control stool specimens which were collected from infants in the same village of Tamooh (near Cairo, Egypt), were analyzed by frequency-pulsed electron-capture gas chromatography (FPEC-GC). Watery stools and formed stools, to which distilled water was added, were centrifuged, and the supernatant was extracted with organic solvents and derivatized with specific functional group reagents to form electron-absorbing derivatives of carboxylic acids, hydroxy acids, alcohols, and amines. Results from the study showed distinct differences in FPEC-GC profiles of stools positive for K. pneumoniae, S. liquefaciens, and P. mirabilis. The major differences found were that diarrheal stools from which K. pneumoniae was isolated contained acetoin, a hydroxy acid-labeled peak F, and an unidentified amine, peak A. S. liquefaciens diarrheal stools had FPEC-GC profiles like the controls with the exception that an amine, peak A, was detected. The diarrhel stools containing P. mirabilis produced a distinct amine profile.