The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis?
Eur J Clin Microbiol Infect Dis
; 39(10): 1997-2000, 2020 Oct.
Article
em En
| MEDLINE
| ID: mdl-32462501
ABSTRACT
Several commercial assays for SARS-CoV-2 RT-PCR are available but few of them were assessed. We evaluate the Allplex 2019-nCoV (Seegene) assay using 41 nasopharyngeal samples. The rates of agreement were 92.7% and 100% with the GeneFinder COVID-19 plus (Elitech) and the diagnosis of the infectious disease specialist respectively. Four samples display a Ct < 22.0 for the E and RdRp genes while the N gene was not detected, suggesting a variability of the viral sequence. There was no cross-reactivity with other respiratory viruses. The Allplex 2019-nCoV appears as a reliable method, but additional evaluations using more samples are needed. RT-PCR assays should probably include at least 2 viral targets.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Pneumonia Viral
/
RNA Polimerase Dependente de RNA
/
Proteínas do Envelope Viral
/
Proteínas não Estruturais Virais
/
Infecções por Coronavirus
/
Proteínas do Nucleocapsídeo
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Betacoronavirus
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
Limite:
Humans
País/Região como assunto:
Europa
Idioma:
En
Revista:
Eur J Clin Microbiol Infect Dis
Assunto da revista:
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
França