Consequences of electromagnetic field (EMF) radiation during early pregnancy - androgen synthesis and release from the myometrium of pigs in vitro.
Anim Reprod Sci
; 218: 106465, 2020 Jul.
Article
em En
| MEDLINE
| ID: mdl-32507253
ABSTRACT
An electromagnetic field (EMF) has been found to affect reproductive processes in females. The aim of this study was to determine the effect of low, non-ionizing EMF radiation on the steroidogenic activity of myometrium collected from pigs during the fetal peri-implantation period. Myometrial slices were treated with an EMF (50 and 120â¯Hz, 2 and 4â¯h of incubation) and examined for the aromatase cytochrome P450 17α-hydroxylase/C17-20lyase (CYP17A1) and 3ß-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase (HSD3B1) mRNA transcript abundance, cytochrome P450c17 and 3ßHSD protein abundance and the secretion of androstenedione (A4) and testosterone (T). To determine whether progesterone (P4) functions as a protectant from EMF radiation, the selected slices were treated with P4. In slices incubated without P4, EMF at 50â¯Hz altered cytochrome P450c17 protein abundance (4â¯h), HSD3B1 mRNA transcript abundance (4â¯h) and A4 release (2â¯h) as well as T release (2â¯h) in P4-treated slices. The EMF at 120â¯Hz in non P4-treated slices altered A4 release (2 and 4â¯h) whereas in P4-treated slices altered CYP17A1 mRNA transcript abundance (4â¯h), 3ßHSD protein abundance (4â¯h), A4 (4â¯h) and T release (2â¯h). In conclusion, EMF radiation in the myometrium collected during the peri-implantation period alters the CYP17A1 and HSD3B1 mRNA transcript and encoded protein abundance, and androgen release due to the time of treatment and P4 presence or absence. The P4 did not function directly as an obvious protector against EMF radiation in the myometrium of pigs during the fetal peri-implantation period.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
/
Agentes_cancerigenos
Base de dados:
MEDLINE
Assunto principal:
Suínos
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Campos Eletromagnéticos
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Androgênios
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Miométrio
Limite:
Animals
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Pregnancy
Idioma:
En
Revista:
Anim Reprod Sci
Ano de publicação:
2020
Tipo de documento:
Article