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Bioassay-based detection of Toxoplasma gondii in free-range chickens from Khorramabad, Iran: comparison of direct microscopy and semi-nested PCR.
Alizadeh-Sarabi, Zahra; Pasandideh, Zahra; Shokrani, Hamidreza; Dezfoulian, Omid.
Afiliação
  • Alizadeh-Sarabi Z; Department of Parasitology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran.
  • Pasandideh Z; Department of Parasitology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran.
  • Shokrani H; Department of Parasitology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran. shokrani.hm@lu.ac.ir.
  • Dezfoulian O; Department of Pathology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran.
Mol Biol Rep ; 47(7): 4969-4974, 2020 Jul.
Article em En | MEDLINE | ID: mdl-32577994
This study aimed to investigate the occurrence of anti-Toxoplasma gondii antibodies in free-range chickens from Khorramabad, western Iran, and also to compare the performance of direct microscopy and semi-nested PCR in mice bioassayed with tissues from seropositive chickens. We investigated 97 serum samples from free-range chickens, using the modified agglutination test (MAT). Tissues from all seropositive chickens (MAT ≥ 1:10) were bioassayed in mice. All inoculated mice were examined by direct microscopy and a semi-nested PCR targeting the 529 bp repeat element (RE) of the parasite. Anti-T. gondii antibodies were detected in 21.6% of chicken sera. Eighteen of 21 (85.7%) seropositive chickens were positive in mouse bioassay using molecular DNA detection. However, biological forms of the parasite were isolated only from 11 (52.3%) seropositive chickens. Compared with semi-nested PCR, the sensitivity of direct microscopy was 62.1%. It can be concluded that although direct microscopy is a rapid and specific method for the detection of T. gondii, it does not detect the parasite in all experimentally infected mice. The low sensitivity of direct microscopy highlights the need for molecular techniques, such as RE-based semi-nested PCR, to increase the sensitivity of the mouse bioassay.
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Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Doenças das Aves Domésticas / Galinhas / Toxoplasmose Animal Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Mol Biol Rep Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Irã

Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Doenças das Aves Domésticas / Galinhas / Toxoplasmose Animal Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Mol Biol Rep Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Irã