Detection of colorectal cancer-derived exosomes based on covalent organic frameworks.
Biosens Bioelectron
; 169: 112638, 2020 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-32987328
ABSTRACT
Covalent organic frameworks (COFs) have attracted more and more attention due to their diverse structures and multifunctionality. Their unique physicochemical properties make them exhibit great application potential in the field of biosensing. In this work, we have designed and fabricated a novel COFs-based nanoprobe named HRP-pSC4-AuNPs@COFs, where spherical COFs are functionalized with para-sulfocalix [4] arene hydrate (pSC4)-modified gold nanoparticles (AuNPs) and horseradish peroxidase (HRP). Then, we have applied it for the electrochemical detection of colorectal cancer (CRC)-derived exosomes. In this design, pSC4 as amicable linker can recognize and bind with various amino acid residues on the exosomes surface, while AuNPs with excellent conductivity can accelerate the migration of charge carriers and improve the response of biosensors. Noteworthy, the high porosity of COFs allows them to load a large amount of HRP, endowing COF with high catalytic activity. Meanwhile, the exoskeleton of COFs can maintain the functionality of HRP with significantly elevated stability. With such design, the proposed method shows excellent analytical performance for the detection of CRC-derived exosomes in the linear range from 5 × 102 to 107 particles/µL with a detection limit down to 160 particles/µL. Further, this method has also been used to analyze clinical serum sample, and can successfully distinguish CRC patients from healthy people, indicating the promising potential in clinical diagnosis.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
/
Tipos_de_cancer
/
Colon_e_reto
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Neoplasias Colorretais
/
Nanopartículas Metálicas
/
Exossomos
/
Estruturas Metalorgânicas
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Biosens Bioelectron
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2020
Tipo de documento:
Article