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ARP2/3-independent WAVE/SCAR pathway and class XI myosin control sperm nuclear migration in flowering plants.
Ali, Mohammad Foteh; Fatema, Umma; Peng, Xiongbo; Hacker, Samuel W; Maruyama, Daisuke; Sun, Meng-Xiang; Kawashima, Tomokazu.
Afiliação
  • Ali MF; Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546-0312.
  • Fatema U; Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546-0312.
  • Peng X; State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, 430072 Wuhan, China.
  • Hacker SW; Agriculture and Medical Biotechnology Program, University of Kentucky, Lexington, KY 40546-0312.
  • Maruyama D; Kihara Institute for Biological Research, Yokohama City University, 244-0813 Yokohama, Kanagawa, Japan.
  • Sun MX; State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, 430072 Wuhan, China.
  • Kawashima T; Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY 40546-0312; tomo.k@uky.edu.
Proc Natl Acad Sci U S A ; 117(51): 32757-32763, 2020 12 22.
Article em En | MEDLINE | ID: mdl-33288691
After eukaryotic fertilization, gamete nuclei migrate to fuse parental genomes in order to initiate development of the next generation. In most animals, microtubules control female and male pronuclear migration in the zygote. Flowering plants, on the other hand, have evolved actin filament (F-actin)-based sperm nuclear migration systems for karyogamy. Flowering plants have also evolved a unique double-fertilization process: two female gametophytic cells, the egg and central cells, are each fertilized by a sperm cell. The molecular and cellular mechanisms of how flowering plants utilize and control F-actin for double-fertilization events are largely unknown. Using confocal microscopy live-cell imaging with a combination of pharmacological and genetic approaches, we identified factors involved in F-actin dynamics and sperm nuclear migration in Arabidopsis thaliana (Arabidopsis) and Nicotiana tabacum (tobacco). We demonstrate that the F-actin regulator, SCAR2, but not the ARP2/3 protein complex, controls the coordinated active F-actin movement. These results imply that an ARP2/3-independent WAVE/SCAR-signaling pathway regulates F-actin dynamics in female gametophytic cells for fertilization. We also identify that the class XI myosin XI-G controls active F-actin movement in the Arabidopsis central cell. XI-G is not a simple transporter, moving cargos along F-actin, but can generate forces that control the dynamic movement of F-actin for fertilization. Our results provide insights into the mechanisms that control gamete nuclear migration and reveal regulatory pathways for dynamic F-actin movement in flowering plants.
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Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Nicotiana / Miosinas / Arabidopsis / Proteínas de Arabidopsis / Complexo 2-3 de Proteínas Relacionadas à Actina Tipo de estudo: Prognostic_studies Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Nicotiana / Miosinas / Arabidopsis / Proteínas de Arabidopsis / Complexo 2-3 de Proteínas Relacionadas à Actina Tipo de estudo: Prognostic_studies Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2020 Tipo de documento: Article