Flow cytometry-based ex vivo murine NK cell cytotoxicity assay.
STAR Protoc
; 2(1): 100262, 2021 03 19.
Article
em En
| MEDLINE
| ID: mdl-33490978
ABSTRACT
Direct killing of diseased cells is a hallmark function of NK cells. This protocol describes a flow-based assay to measure in vivo activated murine NK cells' ability to kill target cells ex vivo. Existing published protocols for assaying ex vivo NK cell killing utilized the radioactive chromium release assay or were designed for human NK cells. This protocol details specifically an ex vivo cytotoxicity assay using primary murine NK cells enriched from splenocytes that were activated in vivo with poly(IC). For complete details on the use and execution of this protocol, please refer to Wagner et al. (2020).
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Baço
/
Testes Imunológicos de Citotoxicidade
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Células Matadoras Naturais
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Citotoxicidade Imunológica
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Citometria de Fluxo
Limite:
Animals
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Humans
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Estados Unidos