Expression of MAdCAM-1 and Gut-homing T Cells in Inflamed Pouch Mucosa.
J Crohns Colitis
; 15(9): 1491-1499, 2021 Sep 25.
Article
em En
| MEDLINE
| ID: mdl-33675360
ABSTRACT
BACKGROUND AND AIMS:
Pouchitis is a common complication following formation of an ileal pouch-anal anastomosis [IPAA] after proctocolectomy for ulcerative colitis [UC]. Gut-specific lymphocyte trafficking mechanisms have been identified as players in the pathogenesis of UC. In the present study, we aimed to characterise the presence of lymphocyte subsets expressing gut-homing molecules in pouches and peripheral blood of UC patients with and without pouchitis.METHODS:
Biopsy samples and peripheral blood were collected from 29 patients with an IPAA [seven with active inflammation, 22 without inflammation]. Expression of adhesion molecule MAdCAM-1 was assessed using immunohistochemistry, and flow cytometry was used to characterise expression of integrin α4ß7, C-chemokine receptor 9 [CCR9], and CD103 on T cell subsets.RESULTS:
MAdCAM-1 expression was significantly increased in case of active inflammation in the pouch. T cells expressing integrin α4ß7 were abundant in the pouch mucosa, but the frequency of integrin α4ß7-expressing T cells was decreased on CD4+ lymphocytes during inflammation. Co-expression of gut-homing markers CCR9 and α4ß7 was more pronounced in biopsies compared with peripheral blood, but was not enhanced upon active inflammation.CONCLUSIONS:
Gut-homing T cells are abundant in pouch mucosa, but the classic hypothesis that the chronic inflammatory state is maintained by an accumulation of α4ß7-expressing effector T cells is not supported by our data.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Colite Ulcerativa
/
Moléculas de Adesão Celular
/
Integrinas
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Proctocolectomia Restauradora
/
Pouchite
/
Mucoproteínas
Tipo de estudo:
Etiology_studies
/
Incidence_studies
/
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
J Crohns Colitis
Assunto da revista:
GASTROENTEROLOGIA
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Holanda