Expression and purification of soluble and active human enterokinase light chain in <i>Escherichia coli</i>.

Kim, Young Su; Lee, Hye-Jeong; Park, Sang-Hyun; Kim, Yeu-Chun; Ahn, Jungoh

Expression and purification of soluble and active human enterokinase light chain in Escherichia coli.

Kim, Young Su; Lee, Hye-Jeong; Park, Sang-Hyun; Kim, Yeu-Chun; Ahn, Jungoh.

Afiliação

Kim YS; Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 34141, Republic of Korea.
Lee HJ; Biotechnology Process Engineering Center, KRIBB, Cheongju 28116, Republic of Korea.
Park SH; Biotechnology Process Engineering Center, KRIBB, Cheongju 28116, Republic of Korea.
Kim YC; Biotechnology Process Engineering Center, KRIBB, Cheongju 28116, Republic of Korea.
Ahn J; Department of Bioprocess Engineering, KRIBB School of Biotechnology, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea.

Biotechnol Rep (Amst) ; 30: e00626, 2021 Jun.

Article em En | MEDLINE | ID: mdl-34026576

Palavras-chave

D4K, (Aspartic Acid)4 Lysine; EK, enterokinase; Escherichia coli; Fusion technology; Human enterokinase light chain; IPTG, isopropyl ß-D-1-thiogalactopyranoside; MBP, maltose-binding protein; Recombinant protein; Self-cleavage; TEV, tobacco etch virus; bEKL, bovine enterokinase light chain; hEKL, human enterokinase light chain

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Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Idioma: En Revista: Biotechnol Rep (Amst) Ano de publicação: 2021 Tipo de documento: Article

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