Carbazole alkaloids with potential cytotoxic activities targeted on PCK2 protein from Murraya microphylla.
Bioorg Chem
; 114: 105113, 2021 09.
Article
em En
| MEDLINE
| ID: mdl-34175718
ABSTRACT
From the 95% aqueous ethanol extract of Murraya microphylla, five pairs of new carbazole alkaloid enantiomers, (+/-)-microphylines N-R (1a/1b-5a/5b), were isolated, together with 20 known carbazole alkaloids. The structures of the new compounds were determined by the HRMS and NMR spectroscopic data, along with the calculated electronic circular dichroism (ECD) and Mo2(AcO)4-induced CD data. The known compound (+)-mahanine (21) showed significant cytotoxicities against Du145, HepG2, HeLa, and HCT-116 cell lines, and its possible mechanism was deduced to target on phosphoenolpyruvate carboxykinase 2 (PCK2) protein via surface plasmon resonance (SPR) and molecular docking.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
/
Prevencao_e_fatores_de_risco
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Alcoolismo
Base de dados:
MEDLINE
Assunto principal:
Carbazóis
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Fosfoenolpiruvato Carboxiquinase (ATP)
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Murraya
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Alcaloides
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Inibidores Enzimáticos
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Antineoplásicos
Limite:
Humans
Idioma:
En
Revista:
Bioorg Chem
Ano de publicação:
2021
Tipo de documento:
Article