Your browser doesn't support javascript.
loading
Equine infection with Leishmania spp. in Costa Rica: Study of five cases.
Ortega-García, María Victoria; Salguero, Francisco Javier; García, Nerea; Domínguez, Mercedes; Moreno, Inmaculada; Berrocal, Alexis.
Afiliação
  • Ortega-García MV; Subdirección de Sistemas Terrestres, Instituto Nacional de Técnica Aeroespacial (INTA)-Campus La Marañosa, Madrid, Spain.
  • Salguero FJ; Department of Pathology and infectious Diseases, University of Surrey, Guildford, UK.
  • García N; Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense de Madrid, Madrid, Spain.
  • Domínguez M; Unidad de Inmunología Microbiana, Área de Inmunología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
  • Moreno I; Unidad de Inmunología Microbiana, Área de Inmunología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
  • Berrocal A; Histopatovet, Heredia, Costa Rica.
Vet Med Sci ; 7(6): 2234-2239, 2021 11.
Article em En | MEDLINE | ID: mdl-34342158
BACKGROUND: Cutaneous forms of leishmaniosis due to Leishmania braziliensis have been reported in horses in the New World. Domestic animals play a role in the transmission of the disease. In Costa Rica, human cases of L. braziliensis, L. panamensis and L. infantum have been reported. OBJECTIVES: The present report describes five cases of equine cutaneous leishmaniosis in Costa Rica. The aetiological diagnosis was based on the presence of the parasite within the lesions. METHODS: Skin biopsies were used to perform histopathological analyses of the lesions. Immunohistochemistry was used to detect the presence of the Leishmania spp. antigens in tissue sections. Laser-capture micro-dissection and quantitative real-time PCR techniques were carried out to detect the pathogen nucleic acid within the microscopic lesions. RESULTS: Histopathological analyses showed a granulomatous inflammation within the dermis, with multi-nucleated giant cells, macrophages, lymphocytes and few neutrophils and eosinophils. We detected the parasite by immunohistochemistry, using a rabbit polyclonal antibody raised against Leishmania spp. However, we could not identify Leishmania spp. by quantitative real-time PCR in formalin-fixed paraffin-embedded tissues, using specific primers for the conserved region in the minicircle of the Leishmania DNA kinetoplast. CONCLUSIONS: Our results emphasise the importance of Leishmania spp. not only as a causative agent of equine cutaneous disease in the New World, but also as a possible emerging pathogen. Leishmaniosis is one of the most prevalent parasitic public health problems worldwide, and equines may have a role in the epidemiology of the disease.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Leishmaniose Cutânea / Doenças dos Cavalos / Leishmania Limite: Animals País/Região como assunto: America central / Costa rica Idioma: En Revista: Vet Med Sci Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Espanha

Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Leishmaniose Cutânea / Doenças dos Cavalos / Leishmania Limite: Animals País/Região como assunto: America central / Costa rica Idioma: En Revista: Vet Med Sci Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Espanha