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Comparison of the Transcriptomic Signatures in Pediatric and Adult CML.
Youn, Minyoung; Smith, Stephanie M; Lee, Alex Gia; Chae, Hee-Don; Spiteri, Elizabeth; Erdmann, Jason; Galperin, Ilana; Jones, Lara Murphy; Donato, Michele; Abidi, Parveen; Bittencourt, Henrique; Lacayo, Norman; Dahl, Gary; Aftandilian, Catherine; Davis, Kara L; Matthews, Jairo A; Kornblau, Steven M; Huang, Min; Sumarsono, Nathan; Redell, Michele S; Fu, Cecilia H; Chen, I-Ming; Alonzo, Todd A; Eklund, Elizabeth; Gotlib, Jason; Khatri, Purvesh; Sweet-Cordero, E Alejandro; Hijiya, Nobuko; Sakamoto, Kathleen M.
Afiliação
  • Youn M; Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Smith SM; Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Lee AG; Department of Pediatrics, University of California, San Francisco, CA 94143, USA.
  • Chae HD; Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Spiteri E; Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Erdmann J; Cytogenetics Laboratory, Stanford Health Care, Stanford, CA 94304, USA.
  • Galperin I; Cytogenetics Laboratory, Stanford Health Care, Stanford, CA 94304, USA.
  • Jones LM; Cytogenetics Laboratory, Stanford Health Care, Stanford, CA 94304, USA.
  • Donato M; Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Abidi P; Institute for Immunity, Transplantation and Infection, Stanford University, Stanford, CA 94305, USA.
  • Bittencourt H; Institute for Immunity, Transplantation and Infection, Stanford University, Stanford, CA 94305, USA.
  • Lacayo N; Stanford Center for Biomedical Informatics Research, Stanford University, Stanford, CA 94305, USA.
  • Dahl G; Division of Hematology, Department of Medicine, Stanford Cancer Institute, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Aftandilian C; Hematology-Oncology Division, Charles Bruneau Cancer Center, Centre Hospitalier Universitaire Sainte-Justine, Montreal, QC H3T 1C5, Canada.
  • Davis KL; Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Matthews JA; Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Kornblau SM; Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Huang M; Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Sumarsono N; Department of Leukemia, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.
  • Redell MS; Department of Leukemia, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.
  • Fu CH; Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Chen IM; Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Alonzo TA; Division of Pediatric Hematology/Oncology, Baylor College of Medicine, Houston, TX 77030, USA.
  • Eklund E; Division of Hematology/Oncology, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
  • Gotlib J; Department of Pathology, University of New Mexico Comprehensive Cancer Center, Albuquerque, NM 87102, USA.
  • Khatri P; Department of Preventive Medicine, University of Southern California, Los Angeles, CA 90032, USA.
  • Sweet-Cordero EA; Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
  • Hijiya N; Division of Hematology, Department of Medicine, Stanford Cancer Institute, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Sakamoto KM; Institute for Immunity, Transplantation and Infection, Stanford University, Stanford, CA 94305, USA.
Cancers (Basel) ; 13(24)2021 Dec 14.
Article em En | MEDLINE | ID: mdl-34944883
ABSTRACT
Children with chronic myeloid leukemia (CML) tend to present with higher white blood counts and larger spleens than adults with CML, suggesting that the biology of pediatric and adult CML may differ. To investigate whether pediatric and adult CML have unique molecular characteristics, we studied the transcriptomic signature of pediatric and adult CML CD34+ cells and healthy pediatric and adult CD34+ control cells. Using high-throughput RNA sequencing, we found 567 genes (207 up- and 360 downregulated) differentially expressed in pediatric CML CD34+ cells compared to pediatric healthy CD34+ cells. Directly comparing pediatric and adult CML CD34+ cells, 398 genes (258 up- and 140 downregulated), including many in the Rho pathway, were differentially expressed in pediatric CML CD34+ cells. Using RT-qPCR to verify differentially expressed genes, VAV2 and ARHGAP27 were significantly upregulated in adult CML CD34+ cells compared to pediatric CML CD34+ cells. NCF1, CYBB, and S100A8 were upregulated in adult CML CD34+ cells but not in pediatric CML CD34+ cells, compared to healthy controls. In contrast, DLC1 was significantly upregulated in pediatric CML CD34+ cells but not in adult CML CD34+ cells, compared to healthy controls. These results demonstrate unique molecular characteristics of pediatric CML, such as dysregulation of the Rho pathway, which may contribute to clinical differences between pediatric and adult patients.
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Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Idioma: En Revista: Cancers (Basel) Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Idioma: En Revista: Cancers (Basel) Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Estados Unidos