Circ-MKLN1/miR-377-3p/CTGF Axis Regulates the TGF-ß2-induced Posterior Capsular Opacification in SRA01/04 Cells.
Curr Eye Res
; 47(3): 372-381, 2022 03.
Article
em En
| MEDLINE
| ID: mdl-34961410
ABSTRACT
OBJECTIVE:
Posterior capsular opacification (PCO) is a common postoperative ocular complication after cataract surgery. Little research focused on the regulation of circular RNAs (circRNAs) in PCO. This study was designed to investigate the function of circRNA-muskelin (circ-MKLN1) in PCO.METHODS:
SRA01/04 cells were treated with transforming growth factor (TGF)-ß2. Cell viability was analyzed by Cell Counting Kit-8 (CCK-8) assay. Transwell assay was used for cell migration and invasion detection. Cell migration was also measured by wound healing assay. Epithelial-mesenchymal transition (EMT)-related proteins and connective tissue growth factor (CTGF) were quantified using western blot.RESULTS:
Cell viability, migration, invasion and EMT process in SRA01/04 cells were facilitated by TGF-ß2. Circ-MKLN1 expression was enhanced in 17 PCO lens samples relative to 19 normal lens samples and TGF-ß2-treated SRA01/04 cells contrasted to control cells. Downregulation of circ-MKLN1 inhibited the effects of TGF-ß2 on SRA01/04 cells. Circ-MKLN1 targeted miR-377-3p and the regulation of si-circ-MKLN1 for the TGF-ß2-induced influences was related to the upregulation of miR-377-3p. CTGF was the target gene for miR-377-3p. CTGF knockdown also abolished the TGF-ß2-mediated cell growth, migration and invasion of SRA01/04 cells. The function of miR-377-3p was achieved by reducing the CTGF level. TGF-ß2-induced CTGF expression promotion was alleviated by si-circ-MKLN1 through upregulating the expression of miR-377-3p.CONCLUSION:
These results showed that circ-MKLN1 contributed to the progression of PCO in vitro by increasing the CTGF expression via sponging miR-377-3p. Circ-MKLN1 might be important for improving the molecular target therapy in PCO.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
MicroRNAs
/
Opacificação da Cápsula
/
Cristalino
Limite:
Humans
Idioma:
En
Revista:
Curr Eye Res
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China