Cotranslational Mechanisms of Protein Biogenesis and Complex Assembly in Eukaryotes.
Annu Rev Biomed Data Sci
; 5: 67-94, 2022 08 10.
Article
em En
| MEDLINE
| ID: mdl-35472290
ABSTRACT
The formation of protein complexes is crucial to most biological functions. The cellular mechanisms governing protein complex biogenesis are not yet well understood, but some principles of cotranslational and posttranslational assembly are beginning to emerge. In bacteria, this process is favored by operons encoding subunits of protein complexes. Eukaryotic cells do not have polycistronic mRNAs, raising the question of how they orchestrate the encounter of unassembled subunits. Here we review the constraints and mechanisms governing eukaryotic co- and posttranslational protein folding and assembly, including the influence of elongation rate on nascent chain targeting, folding, and chaperone interactions. Recent evidence shows that mRNAs encoding subunits of oligomeric assemblies can undergo localized translation and form cytoplasmic condensates that might facilitate the assembly of protein complexes. Understanding the interplay between localized mRNA translation and cotranslational proteostasis will be critical to defining protein complex assembly in vivo.
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1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Biossíntese de Proteínas
/
Dobramento de Proteína
Idioma:
En
Revista:
Annu Rev Biomed Data Sci
Ano de publicação:
2022
Tipo de documento:
Article