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Ultra-low protein residue of chitosan by one step H2O2 and sodium dodecyl sulfate treatment.
Zhao, Zhongtao; Zhang, Jingxian; Tong, Jun; Yang, Chen; Deng, Hongbing; Du, Yumin; Shi, Xiaowen.
Afiliação
  • Zhao Z; School of Resource and Environmental Science, Hubei Engineering Center of Natural Polymers-Based Medical Materials, Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory, Wuhan University, Wuhan 430079, China.
  • Zhang J; School of Resource and Environmental Science, Hubei Engineering Center of Natural Polymers-Based Medical Materials, Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory, Wuhan University, Wuhan 430079, China.
  • Tong J; School of Resource and Environmental Science, Hubei Engineering Center of Natural Polymers-Based Medical Materials, Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory, Wuhan University, Wuhan 430079, China.
  • Yang C; School of Resource and Environmental Science, Hubei Engineering Center of Natural Polymers-Based Medical Materials, Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory, Wuhan University, Wuhan 430079, China.
  • Deng H; School of Resource and Environmental Science, Hubei Engineering Center of Natural Polymers-Based Medical Materials, Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory, Wuhan University, Wuhan 430079, China.
  • Du Y; School of Resource and Environmental Science, Hubei Engineering Center of Natural Polymers-Based Medical Materials, Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory, Wuhan University, Wuhan 430079, China.
  • Shi X; School of Resource and Environmental Science, Hubei Engineering Center of Natural Polymers-Based Medical Materials, Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory, Wuhan University, Wuhan 430079, China. Electronic address: shixw@whu.edu.cn.
Int J Biol Macromol ; 222(Pt B): 2977-2986, 2022 Dec 01.
Article em En | MEDLINE | ID: mdl-36240890
Residual protein in chitosan-based biomaterials may cause inflammation, allergy, and immune rejection after surgery, impeding their clinical application. Facile production of chitosan with ultra-low protein content (residual protein <0.2 %) is yet to be addressed. Herein, we proposed a one-step method for preparing chitosan with residue protein content <0.2 % by using hydrogen peroxide and sodium dodecyl sulfate, which is simple, time-saving, cost-effective, and acid/alkali-free. Notably, the molecular weight of chitosan can be reduced simultaneously. The effects of experimental parameters (i.e. hydrogen peroxide concentration (0.01 %-1 %), SDS concentration (5 %-20 %), and reaction temperature (50 °C-70 °C)) on the protein removal and molecular weight decrease were systematically analyzed by response surface methodology. The results show that temperature and H2O2 concentration are the main parameters affecting the deproteinization of chitosan. Further characterizations on the resulting ultra-low protein residue chitosan revealed unchanged chemical structure, enhanced crystallinity, and reduced thermal stability. The proposed one-step deproteination method may have great potential for industrial mass production of ultra-low protein residue chitosan.
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Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Quitosana Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Quitosana Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China