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Targeted Proteomic Analysis of Small GTPases in Radioresistant Breast Cancer Cells.
Gao, Zi; Yang, Yen-Yu; Huang, Ming; Qi, Tianyu F; Wang, Handing; Wang, Yinsheng.
Afiliação
  • Gao Z; Department of Chemistry, University of California Riverside, Riverside, California92521-0403, United States.
  • Yang YY; Department of Chemistry, University of California Riverside, Riverside, California92521-0403, United States.
  • Huang M; Environmental Toxicology Graduate Program, University of California Riverside, Riverside, California92521-0403, United States.
  • Qi TF; Environmental Toxicology Graduate Program, University of California Riverside, Riverside, California92521-0403, United States.
  • Wang H; Department of Chemistry, University of California Riverside, Riverside, California92521-0403, United States.
  • Wang Y; Department of Chemistry, University of California Riverside, Riverside, California92521-0403, United States.
Anal Chem ; 94(43): 14925-14930, 2022 11 01.
Article em En | MEDLINE | ID: mdl-36264766
Radiation therapy benefits more than 50% of all cancer patients and cures 40% of them, where ionizing radiation (IR) deposits energy to cells and tissues, thereby eliciting DNA damage and resulting in cell death. Small GTPases are a superfamily of proteins that play critical roles in cell signaling. Several small GTPases, including RAC1, RHOB, and RALA, were previously shown to modulate radioresistance in cancer cells. However, there is no systematic proteomic study on small GTPases that regulate radioresistance in cancer cells. Herein, we applied a high-throughput scheduled multiple-reaction monitoring (MRM) method, along with the use of synthetic stable isotope-labeled (SIL) peptides, to identify differentially expressed small GTPase proteins in two pairs of breast cancer cell lines, MDA-MB-231 and MCF7, and their corresponding radioresistant cell lines. We identified 7 commonly altered small GTPase proteins with over 1.5-fold changes in the two pairs of cell lines. We also discovered ARFRP1 as a novel regulator of radioresistance, where its downregulation promotes radioresistance in breast cancer cells. Together, this represents the first comprehensive investigation about the differential expression of the small GTPase proteome associated with the development of radioresistance in breast cancer cells. Our work also uncovered ARFRP1 as a new target for enhancing radiation sensitivity in breast cancer.
Assuntos

Texto completo: 1 Coleções: 01-internacional Temas: Geral / Tipos_de_cancer / Outros_tipos Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / Proteínas Monoméricas de Ligação ao GTP Limite: Female / Humans Idioma: En Revista: Anal Chem Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Temas: Geral / Tipos_de_cancer / Outros_tipos Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / Proteínas Monoméricas de Ligação ao GTP Limite: Female / Humans Idioma: En Revista: Anal Chem Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos