A Novel Cell Volume Sensor for Real-Time Analysis of Ca2+-Activated K+ Channel.
ACS Biomater Sci Eng
; 9(9): 5255-5259, 2023 09 11.
Article
em En
| MEDLINE
| ID: mdl-37639544
ABSTRACT
Potassium channels play a vital role in cell volume regulation. A cell volume sensor was constructed by integrating regulatory volume decrease (RVD) with quartz-crystal microbalance (QCM) for studying potassium channels and their expression. The sensor successfully monitored the K+ channel's activities during RVD by sensitive and noninvasive means. It showed that Ca2+ activated the K+ channel (KCa) and enhanced the RVD level. The inhibition of blockers on K+ channels exhibited an obvious difference in RVD level between normal and cancerous nasopharyngeal cells, suggesting that the KCa channel contributes a dominant role to the RVD function and provides an approach to identify the activation of various K+ channels.
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01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Canais de Potássio
Idioma:
En
Revista:
ACS Biomater Sci Eng
Ano de publicação:
2023
Tipo de documento:
Article