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Suppressing Src-Mediated EGFR Signaling by Sustained Calcium Supply Targeting Triple-Negative Breast Cancer.
Jeong, Keun-Yeong; Park, Seon Young; Park, Min Hee; Kim, Hwan Mook.
Afiliação
  • Jeong KY; Gachon Institute of Pharmaceutical Science, Gachon University, Incheon 21936, Republic of Korea.
  • Park SY; Gachon Institute of Pharmaceutical Science, Gachon University, Incheon 21936, Republic of Korea.
  • Park MH; Gachon Institute of Pharmaceutical Science, Gachon University, Incheon 21936, Republic of Korea.
  • Kim HM; MetiMedi Pharmaceuticals Co., 40, Imi-ro, Uiwang-si 16006, Republic of Korea.
Int J Mol Sci ; 24(17)2023 Aug 27.
Article em En | MEDLINE | ID: mdl-37686097
ABSTRACT
Src is emerging as a promising target in triple-negative breast cancer (TNBC) treatment because it activates survival signaling linked to the epidermal growth factor receptor. In this study, the effect of calcium supply on Src degradation was investigated to confirm underlying mechanisms and anticancer effects targeting TNBC. MDA-MB-231 cells, the TNBC cell line, were used. Calcium supply was feasible through lactate calcium salt (CaLac), and the applicable calcium concentration was decided by changes in the viability with different doses of CaLac. Expression of signaling molecules mediated by calcium-dependent Src degradation was observed by Western blot analysis and immunocytochemistry, and the recovery of the signaling molecules was confirmed following calpeptin treatment. The anticancer effect was investigated in the xenograft animal model. Significant suppression of Src was induced by calcium supply, followed by a successive decrease in the expression of epithelial growth factor receptor, RAS, extracellular signal-regulated kinase, and nuclear factor kappa B. Then, the suppression of cyclooxygenase-2 contributed to a significant deactivation of the prostaglandin E2 receptors. These results suggest that calcium supply has the potential to reduce the risk of TNBC. However, as this study is at an early stage to determine clinical applicability, close consideration is needed.
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Texto completo: 1 Coleções: 01-internacional Temas: Geral / Tipos_de_cancer / Outros_tipos Base de dados: MEDLINE Assunto principal: Cálcio / Neoplasias de Mama Triplo Negativas Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Int J Mol Sci Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Temas: Geral / Tipos_de_cancer / Outros_tipos Base de dados: MEDLINE Assunto principal: Cálcio / Neoplasias de Mama Triplo Negativas Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Int J Mol Sci Ano de publicação: 2023 Tipo de documento: Article