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Screening of endophytic fungi from Antarctic mosses: Potential production for L-asparaginase free of glutaminase and urease activity.
Afonso Kessler de Andrade, Guilherme; de Vargas, Maria Victória Magalhães; Goulart, Sara Navarrete Bohi; Bernardes, Bruna Mota; Bezerra, Jadson D P; Lemos, Rafael Plá Matielo; de Carvalho Victoria, Filipe; de Albuquerque, Margéli Pereira.
Afiliação
  • Afonso Kessler de Andrade G; Núcleo de Estudos da Vegetação Antártica, Universidade Federal do Pampa, Rua Aluízio Barros Macedo, s/n. BR 290 - Km 423, São Gabriel, RS 97300-930, Brazil.
  • de Vargas MVM; Núcleo de Estudos da Vegetação Antártica, Universidade Federal do Pampa, Rua Aluízio Barros Macedo, s/n. BR 290 - Km 423, São Gabriel, RS 97300-930, Brazil.
  • Goulart SNB; EcoHealth Lab, Department of Biological Sciences, Texas Tech University, 2500 Broadway, Lubbock, TX 79409, United States.
  • Bernardes BM; Núcleo de Estudos da Vegetação Antártica, Universidade Federal do Pampa, Rua Aluízio Barros Macedo, s/n. BR 290 - Km 423, São Gabriel, RS 97300-930, Brazil.
  • Bezerra JDP; Laboratório de Micologia, Departamento de Biociências e Tecnologia, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO 74605-050, Brazil.
  • Lemos RPM; Núcleo de Estudos da Vegetação Antártica, Universidade Federal do Pampa, Rua Aluízio Barros Macedo, s/n. BR 290 - Km 423, São Gabriel, RS 97300-930, Brazil.
  • de Carvalho Victoria F; Núcleo de Estudos da Vegetação Antártica, Universidade Federal do Pampa, Rua Aluízio Barros Macedo, s/n. BR 290 - Km 423, São Gabriel, RS 97300-930, Brazil. Electronic address: filipevictoria@unipampa.edu.br.
  • de Albuquerque MP; Núcleo de Estudos da Vegetação Antártica, Universidade Federal do Pampa, Rua Aluízio Barros Macedo, s/n. BR 290 - Km 423, São Gabriel, RS 97300-930, Brazil; Programa Antártico Brasileiro-PROANTAR, Esplanada dos Ministérios, Brasília 70055-900, Brazil.
J Biotechnol ; 377: 1-12, 2023 Nov 20.
Article em En | MEDLINE | ID: mdl-37806388
Studies involving endophytic fungi aim to identify organisms inhabiting extreme and relatively unexplored environments, as these fungi possess unique characteristics and uncommon biochemical pathways that enable them to produce compounds with biotechnological potential. Among various enzymes, L-Asparaginase is employed in the treatment of Acute Lymphoblastic Leukemia. In this study, we identified endophytic fungi from Sanionia uncinata and Polytrichastrum alpinum collected on King George Island in Antarctica. The fungi were categorized into morphological groups based on their characteristics, molecularly identified, and assessed for L-Asparaginase (L-ASNase) enzyme production. Subsequently, production optimization was conducted. A total of 161 endophytes were isolated from 504 moss gametophytes, with 107 originating from P. alpinum and 54 from S. uncinata. These isolates were categorized into 31 morphotypes. Fungi exhibiting high enzyme production were identified molecularly. Among them, nine identified isolates belonged to the genera Aspergillus, Collariella, Diaporthe, Epicoccum, Peroneutypa, Xylaria, and Trametes. Three of these isolates were identified at the species level through multigene phylogeny, namely Epicoccum nigrum, Collariella virescens, and Peroneutypa scoparia. All 31 fungi were subjected to solid media testing for L-ASNase enzyme production, with 22 isolates demonstrating production capability, and 13 of them produced L-ASNase free from Urease and Glutaminase. The isolates displaying solid media production underwent further testing in liquid media, all of which exhibited enzyme production ranging from 0.75 to 1.29 U g-1. Notably, the three fungi identified at the species level were the highest producers of the enzyme (1.29, 1.17, and 1.13 U g-1). The production of these fungi was optimized using the Taguchi method, resulting in production values ranging from 0.687 to 2.461 U g-1. In conclusion, our findings indicate that Antarctic moss endophytic fungi exhibit significant potential for the production of the anti-leukemic enzyme L-ASNase.
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Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Briófitas Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Idioma: En Revista: J Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Briófitas Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Idioma: En Revista: J Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2023 Tipo de documento: Article