Photocurrent Polarity Reversal Induced by Electron-Donor Release for the Highly Sensitive Photoelectrochemical Detection of Vascular Endothelial Growth Factor 165.
Anal Chem
; 95(44): 16392-16397, 2023 11 07.
Article
em En
| MEDLINE
| ID: mdl-37885198
ABSTRACT
Photocurrent polarity reversal is a switching process between the anodic and cathodic pathways and is critical for eliminating false positivity and improving detection sensitivity in photoelectrochemical (PEC) sensing. In this study, we construct a PEC sensor with excellent photocurrent polarity reversal induced by ascorbic acid (AA) as an electron donor with the energy level matching the photoactive material zirconium metal-organic framework (ZrMOF). The ZrMOF-modified electrode demonstrates cathodic photocurrent in the presence of O2 as an electron acceptor, while the anodic photocurrent is generated in the presence of AA, achieving photocurrent polarity reversal. By the in situ release of AA from AA-encapsulated apoferritin modified with DNA 2 (AA@APO-S2) as a detection tag in the presence of trypsin after the recognition of hairpin DNA-modified indium tin oxide to the reaction product of aptamer/DNA 1 with the target protein and the following rolling cycle amplification for introducing the detection tag to the sensing interface, the reversed photocurrent shows an enhanced photocurrent response to the target protein, leading to a highly sensitive PEC sensing strategy. This strategy realizes the detection of vascular endothelial growth factor 165 with good specificity, a wide linear range, and a low detection limit down to 5.3 fM. The actual sample analysis offers the detection results of the proposed PEC sensor comparable to those of commercial enzyme-linked immunosorbent assay tests, indicating the promising application of the photocurrent polarity reversal-based PEC sensing strategy in biomolecule detection and clinical diagnosis.
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Aptâmeros de Nucleotídeos
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2023
Tipo de documento:
Article