Elevated levels of peripheral Th17 cells and Th17-related cytokines in patients with periampullary adenocarcinoma.
Hum Immunol
; 85(1): 110748, 2024 Jan.
Article
em En
| MEDLINE
| ID: mdl-38177009
ABSTRACT
AIM:
Periampullary adenocarcinoma (PAC) is a malignant tumor originating at the ampulla of Vater, distal common bile duct, head of the pancreas, ampulla and duodenum. The levels of circulating Th17 cells and Th17-related cytokines in patients with PAC remain unreported. Therefore, the aim of this study was to determine the levels of circulating Th17 cells and Th17-related cytokines in patients with PAC. MATERIALS ANDMETHODS:
Flow cytometry was used to measure Th17 cell proportions in PBMCs from 60 PAC patients and 30 healthy controls. Enzyme-linked immunosorbent assay (ELISA) was used to quantify IL-17A and IL-23 levels in serum samples, while quantitative reverse transcription polymerase chain reaction (qRT-PCR) assessed IL-17A mRNA expression and Th17-related transcription factors (RORγt and STAT3) in tissue samples.RESULTS:
The findings showed a substantial increase in Th17 cell percentages, elevated concentrations of IL-17A and IL-23, and higher mRNA expression levels of IL-17A, RORγt, and STAT3 in patients with PAC when compared to healthy controls (HCs).CONCLUSION:
Th17 cells play an important role in the pathogenesis of PAC and may represent potential therapeutic targets.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
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Tipos_de_cancer
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Outros_tipos
Base de dados:
MEDLINE
Assunto principal:
Adenocarcinoma
/
Citocinas
Limite:
Humans
Idioma:
En
Revista:
Hum Immunol
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Índia