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Thiolation-Based Protein-Protein Hydrogels for Improved Wound Healing.
Liu, Xing; Guo, Zhao; Wang, Jie; Shen, Wenting; Jia, Zhenzhen; Jia, Shuang; Li, Limiao; Wang, Jieqi; Wang, Liping; Li, Jiaqi; Sun, Yinan; Chen, Yufang; Zhang, Min; Bai, Jia; Wang, Liyao; Li, Xinyu.
Afiliação
  • Liu X; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010020, P .R. China.
  • Guo Z; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010020, P .R. China.
  • Wang J; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010020, P .R. China.
  • Shen W; Shandong Provincial Key Laboratory of Animal Resistance Biology, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Science, Shandong Normal University, Jinan, 250014, P. R. China.
  • Jia Z; Shandong Provincial Key Laboratory of Animal Resistance Biology, Key Laboratory of Food Nutrition and Safety of Shandong Normal University, College of Life Science, Shandong Normal University, Jinan, 250014, P. R. China.
  • Jia S; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010020, P .R. China.
  • Li L; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010020, P .R. China.
  • Wang J; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010020, P .R. China.
  • Wang L; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010020, P .R. China.
  • Li J; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010020, P .R. China.
  • Sun Y; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010020, P .R. China.
  • Chen Y; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010020, P .R. China.
  • Zhang M; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010020, P .R. China.
  • Bai J; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010020, P .R. China.
  • Wang L; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010020, P .R. China.
  • Li X; State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia Key Laboratory for Molecular Regulation of the Cell, Institute of Biomedical Sciences, School of Life Sciences, Inner Mongolia University, Hohhot, 010020, P .R. China.
Adv Healthc Mater ; 13(14): e2303824, 2024 06.
Article em En | MEDLINE | ID: mdl-38303578
ABSTRACT
The limitations of protein-based hydrogels, including their insufficient mechanical properties and restricted biological functions, arise from the highly specific functions of proteins as natural building blocks. A potential solution to overcome these shortcomings is the development of protein-protein hydrogels, which integrate structural and functional proteins. In this study, a protein-protein hydrogel formed by crosslinking bovine serum albumin (BSA) and a genetically engineered intrinsically disordered collagen-like protein (CLP) through Ag─S bonding is introduced. The approach involves thiolating lysine residues of BSA and crosslinking CLP with Ag+ ions, utilizing thiolation of BSA and the free-cysteines of CLP. The resulting protein-protein hydrogels exhibit exceptional properties, including notable plasticity, inherent self-healing capabilities, and gel-sol transition in response to redox conditions. In comparison to standalone BSA hydrogels, these protein-protein hydrogels demonstrate enhanced cellular viability, and improved cellular migration. In vivo experiments provide conclusive evidence of accelerated wound healing, observed not only in murine models with streptozotocin (Step)-induced diabetes but also in zebrafish models subjected to UV-burn injuries. Detailed mechanistic insights, combined with assessments of proinflammatory cytokines and the expression of epidermal differentiation-related proteins, robustly validate the protein-protein hydrogel's effectiveness in promoting wound repair.
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Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Cicatrização / Peixe-Zebra / Soroalbumina Bovina / Hidrogéis Limite: Animals Idioma: En Revista: Adv Healthc Mater Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Temas: Geral Base de dados: MEDLINE Assunto principal: Cicatrização / Peixe-Zebra / Soroalbumina Bovina / Hidrogéis Limite: Animals Idioma: En Revista: Adv Healthc Mater Ano de publicação: 2024 Tipo de documento: Article