Development and evaluation of test methods for the detection and enumeration of opportunistic waterborne pathogens from the hospital environment.
J Hosp Infect
; 149: 98-103, 2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-38685413
ABSTRACT
BACKGROUND:
Many Gram-negative bacteria other than Pseudomonas aeruginosa have been implicated in waterborne outbreaks, but standardized laboratory detection methods for these organisms have not been established.AIM:
This study aimed to establish laboratory testing methodologies for six waterborne pathogens Acinetobacter spp., Burkholderia spp., Cupriavidus spp., Delftia acidovorans, Elizabethkingia spp. and Stenotrophomonas maltophilia.METHODS:
Water samples were spiked by UK Health Security Agency laboratories and sent to the Glasgow Royal Infirmary laboratory for analysis. Water samples were spiked with either a pure culture of target organism or the target organism in water containing normal background flora, to ensure that the methodology could identify organisms from a mixed culture. Volumes of 100 mL were filtered under negative pressure on to culture media and incubated at 30 °C and 37 °C. The incubation time was 7 days, with plates read on days 2, 5 and 7. Further identification of colonies was undertaken using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS).FINDINGS:
Optimal recovery of organisms was obtained by culturing water samples on tryptic soy agar, chocolate bacitracin agar and pseudomonas selective agar. The optimal temperature for isolation was 30 °C. The optimal incubation time was 5 days, and MALDI-TOF MS identified all test species reliably.CONCLUSION:
The methodology described was able to detect the six tested waterborne pathogens reliably, and can be utilized by laboratories involved in testing water samples during outbreak investigations.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
Base de dados:
MEDLINE
Assunto principal:
Microbiologia da Água
/
Hospitais
Limite:
Humans
País/Região como assunto:
Europa
Idioma:
En
Revista:
J Hosp Infect
Ano de publicação:
2024
Tipo de documento:
Article