Single-cell profiling reveals the metastasis-associated immune signature of hepatocellular carcinoma.
Immun Inflamm Dis
; 12(5): e1264, 2024 May.
Article
em En
| MEDLINE
| ID: mdl-38780041
ABSTRACT
AIM:
Metastasis is the leading cause of mortality in hepatocellular carcinoma (HCC). The metastasis-associated immune signature in HCC is worth exploring.METHODS:
Bioinformatic analysis was conducted based on the single-cell transcriptome data derived from HCC patients in different stages. Cellular composition, pseudotime state transition, and cell-cell interaction were further analyzed and verified.RESULTS:
Generally, HCC with metastasis exhibited suppressive immune microenvironment, while HCC without metastasis exhibited active immune microenvironment. Concretely, effector regulatory T cells (eTregs) were found to be enriched in HCC with metastasis. PHLDA1 was identified as one of exhaustion-specific genes and verified to be associated with worse prognosis in HCC patients. Moreover, A novel cluster of CCR7+ dendritic cells (DCs) was identified with high expression of maturation and migration marker genes. Pseudotime analysis showed that inhibition of differentiation occurred in CCR7+ DCs rather than cDC1 in HCC with metastasis. Furthermore, interaction analysis showed that the reduction of CCR7+ DCs lead to impaired CCR7/CCL19 interaction in HCC with metastasis.CONCLUSIONS:
HCC with metastasis exhibited upregulation of exhaustion-specific genes of eTregs and inhibition of CCL signal of a novel DC cluster, which added new dimensions to the immune landscape and provided new immune therapeutic targets in advanced HCC.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
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Tipos_de_cancer
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Outros_tipos
Base de dados:
MEDLINE
Assunto principal:
Células Dendríticas
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Carcinoma Hepatocelular
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Análise de Célula Única
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Microambiente Tumoral
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Neoplasias Hepáticas
Limite:
Humans
Idioma:
En
Revista:
Immun Inflamm Dis
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China