Involvement of lysophosphatidic acid (LPA) receptor-mediated signaling in breast cancer cell functions by long-term tamoxifen treatment under hypoxic and estrogen-deprived conditions.
Pathol Res Pract
; 260: 155385, 2024 Aug.
Article
em En
| MEDLINE
| ID: mdl-38875757
ABSTRACT
BACKGROUND:
Tamoxifen (TAM) is a selective estrogen receptor modulator and has anti-estrogenic activity. Breast cancer cells acquire drug resistance to TAM as a consequence of long-term treatment. Lysophosphatidic acid (LPA) receptor-mediated signaling contributes to the promotion of tumor progression. This study aimed to evaluate the role of LPA receptors in the modulation of biological functions by long-term TAM treatment in breast cancer MCF-7 cells under hypoxic and estrogen-deprived conditions.METHODS:
Long-term TAM treated (MCF-TAM) cells were generated from MCF-7 cells. Cells were cultured in estrogen-free medium at 1â¯% O2. LPA receptor expressions were measured by quantitative real-time RT-PCR analysis. Cell motile activity was investigated using Cell Culture Inserts. The CCK-8 kit was used to determine the cell proliferation rate.RESULTS:
LPAR1 and LPAR3 expressions were elevated in MCF-TAM cells. MCF-TAM cell motility was enhanced by culturing at 1â¯% O2, compared with MCF-7 cells. When cells were cultured in estrogen-deprived medium at 1â¯% O2, the cell proliferation rate of MCF-TAM cells was significantly higher than that of MCF-7 cells.CONCLUSION:
These results suggest that LPA receptor-mediated signaling plays an important role in the acquisition of malignant properties in long-term TAM treated MCF-7 cells under hypoxic and estrogen-deprived conditions.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Temas:
Geral
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Tipos_de_cancer
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Outros_tipos
Base de dados:
MEDLINE
Assunto principal:
Tamoxifeno
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Neoplasias da Mama
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Transdução de Sinais
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Movimento Celular
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Antineoplásicos Hormonais
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Receptores de Ácidos Lisofosfatídicos
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Proliferação de Células
Limite:
Female
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Humans
Idioma:
En
Revista:
Pathol Res Pract
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Pathol. res. pract
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Pathology research and practice (Print)
Ano de publicação:
2024
Tipo de documento:
Article